A simple method for direct cloning cDNA sequence that flanks a region of known sequence from total RNA by applying the inverse polymerase chain reaction.

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超能文献

作者信息

Huang S H, Hu Y Y, Wu C H, Holcenberg J

机构信息

Division of Hematology/Oncology, Children Hospital of Los Angeles, CA 90054.

出版信息

Nucleic Acids Res. 1990 Apr 11;18(7):1922. doi: 10.1093/nar/18.7.1922.

DOI:10.1093/nar/18.7.1922

PMID:1692407

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330645/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a5b/330645/e3f6e8305038/nar00191-0238-a.jpg

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