Li Yinli, Zhu Changjiang, Zhu Jichun, Liang Hao, Chen Dong, Zhao Huiling, Liu Bo
Institute of Photo-biophysics, School of Physics and Electronic, Henan University, Kaifeng, 475004 Henan PR China.
College of Software, Henan University, Kaifeng, 475004 Henan PR China.
Chem Cent J. 2014 Dec 10;8(1):71. doi: 10.1186/s13065-014-0071-2. eCollection 2014.
Phospholipid, a main component of cell membrane, has been explored as a model system of the cell membrane and temporary scaffold materials in recent studies. The mechanical properties of phospholipid layers are essentially interesting since it is involved in several biological processes.
Here, the nanomechanical properties such as indentation force, adhesion force and DMT (Derjaguin-Müller-Toporov) modulus of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) Langmuir-Blodgett (LB) films were analyzed layer by layer with Atomic Force Microscope (AFM) under deionized water condition.
The penetration distances in the indentation force curves are equal to the thicknesses of phospholipid films, and the yield forces of DSPC LB films in deionized water are smaller than that of similar lipid films in buffered solutions due to the influence of ions. Moreover, the DMT modulus of upper layer DSPC LB film is different from that of monolayer DSPC LB film due to the influence of their different substrates. Our results suggest that environment such as surrounding ions and substrate will strongly influence the measured nano-mechanical properties of the lipid bilayer, especially that of the down layer. Graphical AbstractA process about the exploration of nanomechanics of DSPC LB film.
磷脂作为细胞膜的主要成分，在最近的研究中已被用作细胞膜的模型系统和临时支架材料。磷脂层的力学性能本质上很有趣，因为它参与了多个生物过程。
在此，利用原子力显微镜（AFM）在去离子水条件下对1,2-二硬脂酰-sn-甘油-3-磷酸胆碱（DSPC）朗缪尔-布洛杰特（LB）膜的纳米力学性能，如压痕力、粘附力和德亚金-米勒-托波罗夫（DMT）模量进行了逐层分析。
压痕力曲线中的穿透距离等于磷脂膜的厚度，由于离子的影响，去离子水中DSPC LB膜的屈服力小于缓冲溶液中类似脂质膜的屈服力。此外，由于上层DSPC LB膜和单层DSPC LB膜的底物不同，其DMT模量也不同。我们的结果表明，诸如周围离子和底物等环境将强烈影响脂质双层的测量纳米力学性能，尤其是下层的性能。图形摘要DSPC LB膜纳米力学探索过程。
