Department of Biomedical Sciences and Biofilms-Research Center for Biointerfaces (BRCB), Faculty of Health and Society , Malmö University , 205 06 Malmö , Sweden.
Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM) , Linköping University , 581 83 Linköping , Sweden.
Langmuir. 2018 Apr 3;34(13):4107-4115. doi: 10.1021/acs.langmuir.8b00226. Epub 2018 Mar 23.
Lipid bilayers, forming the outer barrier of cells, display a wide array of proteins and carbohydrates for modulating interfacial biological interactions. Formed by the spontaneous self-assembly of lipid molecules, these bilayers feature liquid crystalline order, while retaining a high degree of lateral mobility. Studies of these dynamic phenomena have been hampered by the fragility and instability of corresponding biomimetic cell membrane models. Here, we present the construct of a series of oligoethylene glycol-terminated reversible self-assembled monolayers (rSAMs) featuring lipid-bilayer-like fluidity, while retaining air and protein stability and resistance. These robust and ordered layers were prepared by simply immersing a carboxylic acid-terminated self-assembled monolayer into 5-50 μM aqueous ω-(4-ethylene glycol-phenoxy)-α-(4-amidinophenoxy)decane solutions. It is anticipated that this new class of robust and fluidic two-dimensional biomimetic surfaces will impact the design of rugged cell surface mimics and high-performance biosensors.
脂质双层构成了细胞的外部屏障，展示了广泛的蛋白质和碳水化合物，用于调节界面生物相互作用。这些双层由脂质分子的自发自组装形成，具有液晶有序性，同时保持高度的侧向流动性。这些动态现象的研究受到相应仿生细胞膜模型的脆弱性和不稳定性的阻碍。在这里，我们提出了一系列具有脂质双层类似流动性的聚乙二醇末端可逆自组装单层（rSAM）的构建，同时保持空气和蛋白质的稳定性和抗性。这些坚固有序的层是通过将羧酸末端的自组装单层简单地浸入 5-50 μM 的ω-（4-乙二醇-苯氧基）-α-（4-氨二苯氧基）癸烷水溶液中制备的。预计这种新型坚固且流畅的二维仿生表面将影响坚固的细胞表面模拟物和高性能生物传感器的设计。
