Kuppens Maike, George Isabelle, Lewi Liesbeth, Levtchenko Elena, Allegaert Karel
Department of Woman and Child, University Hospitals, Leuven, Belgium.
J Matern Fetal Neonatal Med. 2012 Jul;25(7):978-80. doi: 10.3109/14767058.2011.602144. Epub 2011 Aug 25.
The paradigm that creatinaemia at birth is equal to maternal creatinaemia may also depend upon the quantification technique applied. Paired maternal-neonatal creatinaemia samples in whom Jaffe in both or compensated Jaffe (maternal) and enzymatic quantification (neonate) were applied.
Extreme low birth weight infants in two time intervals were included when paired maternal-neonatal creatinaemia samples were available. In cohort 1 (2000-2005), creatinaemia (mothers and neonates) was based on Jaffe assay. In cohort 2 (2007-2010), maternal creatinaemia was based on compensated Jaffe. In neonates, an enzymatic technique was applied. Unpaired Mann Whitney U, paired Wilcoxon and Bland-Altman were used.
Based on 80 and 52 paired creatinaemia samples, there was no significant difference between maternal (0.80, 0.41-1.6 mg.dl(-1)) and neonatal creatinaemia (0.78, 0.31-1.46 mg.dl(-1)) in cohort 1 while a significant difference (p < 0.001) between maternal (0.6, 0.29-2.24 mg.dl(-1)) and neonatal creatinaemia (0.67, 0.4-2.2 mg.dl(-1)) was observed for cohort 2. Using Bland-Altman, the fit was perfect for cohort 1 (mean diff -0.02 mg.dl(-1)), but not for cohort 2 (-0.08 mg.dl(-1)).
The quantification method affects the paradigm that creatinaemia at birth is similar to maternal creatinaemia. Maternal and neonatal creatinaemia values depend on the method used. Consequently, method-specific reference values are needed.
出生时肌酐血症等于母体肌酐血症这一范例可能也取决于所应用的定量技术。对同时应用Jaffe法（母体和新生儿）或补偿Jaffe法（母体）与酶法（新生儿）进行定量的母体 - 新生儿配对肌酐血症样本进行研究。
当有母体 - 新生儿配对肌酐血症样本时，纳入两个时间段的极低出生体重儿。在队列1（2000 - 2005年）中，肌酐血症（母体和新生儿）基于Jaffe法测定。在队列2（2007 - 2010年）中，母体肌酐血症基于补偿Jaffe法测定。在新生儿中，应用酶法技术。使用非配对Mann Whitney U检验、配对Wilcoxon检验和Bland - Altman分析。
基于80对和52对肌酐血症样本，队列1中母体（0.80，0.41 - 1.6mg·dl⁻¹）和新生儿肌酐血症（0.78，0.31 - 1.46mg·dl⁻¹）之间无显著差异，而队列2中母体（0.6，0.29 - 2.24mg·dl⁻¹）和新生儿肌酐血症（0.67，0.4 - 2.2mg·dl⁻¹）之间观察到显著差异（p < 0.001）。使用Bland - Altman分析，队列1的拟合良好（平均差值 - 0.02mg·dl⁻¹），但队列2则不然（ - 0.08mg·dl⁻¹）。
定量方法影响出生时肌酐血症与母体肌酐血症相似这一范例。母体和新生儿肌酐血症值取决于所使用的方法。因此，需要特定方法的参考值。
