Cancer Institute and Department of Pharmacology, New York University School of Medicine, 522 First Avenue, Smilow Research Building, Room 1211, New York, New York 10016, USA.
EMBO Rep. 2011 Sep 30;12(10):1047-54. doi: 10.1038/embor.2011.159.
We report the identification of yan, an ETS-domain transcription factor belonging to the Drosophila epidermal growth factor receptor (DER) pathway, as an antagonist of the Wingless signalling pathway. We demonstrate that cells lacking yan function in the Drosophila eye show increased Wingless pathway activity, and inhibition of Wingless signalling in yan(-/-) cells rescues the yan mutant phenotype. Biochemical analysis shows that Yan physically associates with Armadillo, a crucial effector of the Wingless pathway, thereby suggesting a direct regulatory mechanism. We conclude that yan represents a new and unsuspected molecular link between the Wingless and DER pathways.
我们报告了 yan 的鉴定，它是一种属于果蝇表皮生长因子受体（DER）途径的 ETS 结构域转录因子，作为 Wingless 信号通路的拮抗剂。我们证明，在果蝇眼中缺乏 yan 功能的细胞显示出增加的 Wingless 途径活性，并且在 yan(-/-)细胞中抑制 Wingless 信号转导可挽救 yan 突变表型。生化分析表明 Yan 与 Armadillo 物理结合，后者是 Wingless 途径的关键效应物，因此暗示了一种直接的调节机制。我们得出结论，yan 代表了 Wingless 和 DER 途径之间新的和意想不到的分子联系。
