Neuro-Bio-Oncology Center, Policlinico di Monza Foundation, University of Turin, 13100 Vercelli, Italy.
J Oncol. 2011;2011:314962. doi: 10.1155/2011/314962. Epub 2011 Aug 18.
Formation of neurospheres (NS) in cultures of glioblastomas (GBMs), with self-renewal, clonogenic capacities, and tumorigenicity following transplantation into immunodeficient mice, may denounce the existence of brain tumor stem cells (BTSCs) in vivo. In sixteen cell lines from resected primary glioblastomas, NS showed the same genetic alterations as primary tumors and the expression of stemness antigens. Adherent cells (AC), after adding 10% of fetal bovine serum (FBS) to the culture, were genetically different from NS and prevailingly expressed differentiation antigens. NS developed from a highly malignant tumor phenotype with proliferation, circumscribed necrosis, and high vessel density. Beside originating from transformed neural stem cells (NSCs), BTSCs may be contained within or correspond to dedifferentiated cells after mutation accumulation, which reacquire the expression of stemness antigens.
神经球（NS）在Glioblastomas（GBMs）的培养中形成，具有自我更新、克隆形成能力，并在移植到免疫缺陷小鼠后具有致瘤性，这可能揭示了体内脑肿瘤干细胞（BTSCs）的存在。在从切除的原发性Glioblastomas 中分离出的 16 个细胞系中，NS 显示出与原发性肿瘤相同的遗传改变，并表达了干细胞抗原。在向培养物中添加 10%的胎牛血清（FBS）后，贴壁细胞（AC）在遗传上与 NS 不同，并且主要表达分化抗原。NS 起源于具有增殖、局灶性坏死和高血管密度的高度恶性肿瘤表型。BTSCs 除了来源于转化的神经干细胞（NSCs）外，还可能包含在突变积累后的去分化细胞内，或者对应于去分化细胞，这些细胞重新获得了干细胞抗原的表达。
