Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Lab Chip. 2011 Oct 21;11(20):3487-92. doi: 10.1039/c1lc20511k. Epub 2011 Aug 25.
This paper reports the investigation of a micro-gas chromatography (μGC) system that utilizes an array of miniaturized motionless Knudsen pumps (KPs) as well as microfabricated separation columns and optical detectors. A prototype system was built to achieve a flow rate of 1 mL min(-1) and 0.26 mL min(-1) for helium and dry air, respectively, when they were used as carrier gas. This system was then employed to evaluate GC performance compromises and demonstrate the ability to separate and detect gas mixtures containing analytes of different volatilities and polarities. Furthermore, the use of pressure programming of the KP array was demonstrated to significantly shorten the analysis time while maintaining a high detection resolution. Using this method, we obtained a high resolution detection of 5 alkanes of different volatilities within 5 min. Finally, we successfully detected gas mixtures of various polarities using a tandem-column μGC configuration by installing two on-column optical detectors to obtain complementary chromatograms.
本文报道了一种微型气相色谱（μGC）系统的研究，该系统利用了一系列微型静态克努森泵（KP）以及微加工分离柱和光学检测器。建立了一个原型系统，当氦气和干燥空气分别用作载气时，其流速分别达到 1 mL min(-1)和 0.26 mL min(-1)。该系统随后用于评估 GC 性能折衷，并展示分离和检测含有不同挥发性和极性分析物的气体混合物的能力。此外，还证明了使用 KP 阵列的压力编程可以显著缩短分析时间，同时保持高检测分辨率。使用这种方法，我们在 5 分钟内成功地对不同挥发性的 5 种烷烃进行了高分辨率检测。最后，我们通过安装两个柱上光学检测器来获得互补的色谱图，成功地使用串联柱 μGC 配置检测了各种极性的气体混合物。
