State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
J Chromatogr A. 2018 Aug 31;1565:130-137. doi: 10.1016/j.chroma.2018.06.036. Epub 2018 Jun 20.
In this paper, a novel embeded elliptic cylindrical posts (ECPs) with large surface area and wide effective width, which could support more stationary phase and decrease the pressure drop, is applied on the micro-fabricated gas chromatographic (μGC) column. Compared with μGC column with cylindrical posts (CPs), the surface area and effective width of μGC column with ECPs are increased by 29% and 30%. Separation experiments are performed under the same head pressure at column inlet: in experiments of separating mixture 1, the column efficiency of the μGC column with ECPs for C9 has a 76% improvement, and the separation resolution between C8 and C9 also has a 34% improvement; in experiments of separating mixture 2, seven kinds of analytes can be identified by the μGC column with ECPs less than ten minutes, while, only six kinds can be identified by the μGC column with CPs in almost the same time. In detail, the column efficiency of the μGC column with ECPs for toluene has a 129% improvement, and the separation resolution between benzene and toluene also has a 56.4% improvement. Hence, the μGC column with the inner structures of ECPs is a valid means to improve column efficiency and resolution in a lower pressure drop.
本文提出了一种新型嵌入式椭圆柱状柱（ECP），其具有较大的表面积和较宽的有效宽度，可支持更多的固定相并降低压降。该结构应用于微结构气相色谱（μGC）柱中。与具有圆柱形柱（CP）的μGC 柱相比，具有 ECP 的μGC 柱的表面积和有效宽度分别增加了 29%和 30%。在相同的柱入口压力下进行分离实验：在分离混合物 1 的实验中，CP 结构的 μGC 柱对 C9 的柱效率提高了 76%，C8 和 C9 之间的分离分辨率也提高了 34%；在分离混合物 2 的实验中，七种分析物可以在不到十分钟的时间内通过具有 ECP 的 μGC 柱来识别，而在几乎相同的时间内，仅可以通过 CP 结构的 μGC 柱来识别六种分析物。具体而言，具有 ECP 的 μGC 柱对甲苯的柱效率提高了 129%，苯和甲苯之间的分离分辨率也提高了 56.4%。因此，具有 ECP 内部结构的 μGC 柱是在较低压降下提高柱效率和分辨率的有效手段。
