Chu R M, Moore D M, Conroy J D
Can J Comp Med. 1979 Jan;43(1):29-38.
Two day old piglets were inoculated intravenously with 1 ml of swine vesicular disease virus UK-G 27-72 isolate. Using infectivity tests, immunofluorescent staining and gross and histopathological examination, pathogenesis of the infection was studied in tissue specimens collected daily from one through seven days postinoculation. Swine vesicular disease virus had a strong affinity for the epithelia of the tongue, snout, coronary band and lips, the myocardium and the lymphoid elements of the tonsil and the brain stem. The virus had the greatest affinity for the epithelium of the tongue. However, there was no evidence that the tongue was the initial replication site for swine vesicular disease virus. Prickle cells in the stratum spinosum appear to be the primary targets for the virus. The necrotic foci in the stratum spinosum appeared first, followed the next day by reticular degeneration and multilocular intraepidermal vesicular formation. In the digestive tract and most of the other visceral organs the short duration and sudden drop of the virus titres and the negative fluorescence and pathological findings suggest that these are not important sites for the replication of swine vesicular disease virus in this experiment. The virus was recovered from most of the central nervous tissue specimens. Although the piglets had significant central nervous system lesions, signs of impaired central nervous system function were not detected. However, subtle nervous signs could have been obscured by difficulties in locomotion resulting from severe lesions of the feet.
给两日龄仔猪静脉注射1毫升猪水疱病病毒英国-G 27 - 72分离株。通过感染性试验、免疫荧光染色以及大体和组织病理学检查，对接种后1至7天每天采集的组织标本中感染的发病机制进行了研究。猪水疱病病毒对舌、口鼻部、冠状带和唇部的上皮、心肌以及扁桃体和脑干的淋巴组织具有很强的亲和力。该病毒对舌上皮的亲和力最大。然而，没有证据表明舌是猪水疱病病毒的初始复制部位。棘层中的棘细胞似乎是该病毒的主要靶细胞。棘层中的坏死灶首先出现，第二天接着是网状变性和多房性表皮内水疱形成。在消化道和大多数其他内脏器官中，病毒滴度的持续时间短和突然下降以及阴性荧光和病理结果表明，在本实验中这些不是猪水疱病病毒复制的重要部位。从大多数中枢神经组织标本中分离出了病毒。虽然仔猪有明显的中枢神经系统病变，但未检测到中枢神经系统功能受损的迹象。然而，由于足部严重病变导致的运动困难可能掩盖了细微的神经症状。
