Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, (CIMAP-CSIR), Kukrail Picnic Spot Road, P.O. CIMAP, Lucknow 226015, India.
Biotechnol Adv. 2012 May-Jun;30(3):461-8. doi: 10.1016/j.biotechadv.2011.08.010. Epub 2011 Aug 17.
Agrobacterium rhizogenes induced hairy root cultures are entering into a new juncture of functional research in generating pharmaceutical lead compounds by bringing about chemical transformations aided through its inherent enzyme resources. Rational utilization of hairy root cultures as highly effective biotransformation systems has come into existence in the last twenty years involving a wide range of plant systems as well as exogenous substrates and diverse chemical reactions. To date, hairy root cultures are preferred over plant cell/callus and suspension cultures as biocatalyst due to their genetic/biochemical stability, hormone-autotrophy, multi-enzyme biosynthetic potential mimicking that of the parent plants and relatively low-cost cultural requirements. The resultant biotransformed molecules, that are difficult to make by synthetic organic chemistry, can unearth notable practical efficacies by acquiring improved physico-chemical properties, bioavailability, lower toxicity and broader therapeutic properties. The present review summarizes the overall reported advances made in the area of hairy root mediated biotransformation of exogenous substrates with regard to their reaction types, plant systems associated, bacterial strains/molecules involved and final product recovery.
发根农杆菌诱导的发状根培养物通过利用其固有酶资源进行化学转化，在生成药物先导化合物的功能研究中进入了一个新的阶段。在过去的二十年中，发根培养物作为高效的生物转化系统得到了合理利用，涉及广泛的植物系统以及外源底物和多种化学反应。迄今为止，发根培养物由于遗传/生化稳定性、激素自养、多酶生物合成潜力模仿亲本植物以及相对较低的培养要求，已被优先用作生物催化剂，而不是植物细胞/愈伤组织和悬浮培养物。通过合成有机化学难以合成的转化分子，可以通过获得改善的物理化学性质、生物利用度、降低的毒性和更广泛的治疗特性来揭示显著的实际疗效。本综述总结了发根介导的外源底物生物转化领域的总体报道进展，涉及反应类型、相关植物系统、涉及的细菌菌株/分子和最终产物回收。
