Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Int J Gynaecol Obstet. 2011 Nov;115(2):175-9. doi: 10.1016/j.ijgo.2011.06.010. Epub 2011 Aug 27.
To determine the distribution of HPV genotypes in cervical neuroendocrine carcinoma (NECA) in northern Thailand, and evaluate the correlation between HPV genotype and clinicopathologic features.
Samples from 111 women treated for cervical NECA at Chiang Mai University Hospital between 1992 and 2009 were tested for HPV genotype. Samples were formaldehyde-fixed, paraffin-embedded, and tested via nested PCR and dot blot hybridization.
Ninety-seven of the 111 samples were adequate for DNA analysis. HPV DNA was detected in 93 samples, of which 76 (81.7%) were single, 14 (15.1%) were multiple, and 3 (3.2%) were untyped infections. HPV18 was the most common subtype (70 cases, 75.3%), followed by HPV16 (28 cases, 30.1%). Other genotypes included HPV58 (3.2%), HPV52 (2.1%), and HPV33 (1.1%). Collectively, HPV16 and/or HPV18 were found in 83 cases (89.3%). Women with HPV18 infection were significantly younger (42.0years) than those with non-HPV18 infections (54.1years) (P=0.003). Associated adenocarcinoma in situ was more frequently seen among women with HPV18 infection (P=0.034).
HPV18 infection was predominant in cervical NECA. Variations in HPV genotype may be related to the clinicopathologic features and pathogenetic pathways of NECA. Vaccination against HPV16 and HPV18 might provide protection against cervical NECA in almost 90% of cases.
确定 HPV 基因型在泰国北部宫颈神经内分泌癌（NECA）中的分布，并评估 HPV 基因型与临床病理特征之间的相关性。
1992 年至 2009 年，在清迈大学医院接受宫颈 NECA 治疗的 111 名女性的样本接受了 HPV 基因型检测。对福尔马林固定、石蜡包埋的样本进行嵌套 PCR 和点印迹杂交检测。
111 个样本中有 97 个样本可用于 DNA 分析。93 个样本检测到 HPV DNA，其中 76 个（81.7%）为单一感染，14 个（15.1%）为多重感染，3 个（3.2%）为未分型感染。HPV18 是最常见的亚型（70 例，75.3%），其次是 HPV16（28 例，30.1%）。其他基因型包括 HPV58（3.2%）、HPV52（2.1%）和 HPV33（1.1%）。HPV16 和/或 HPV18 共在 83 例（89.3%）中检出。HPV18 感染的女性明显比非 HPV18 感染的女性年轻（42.0 岁）（P=0.003）。HPV18 感染与伴发的原位腺癌更常见相关（P=0.034）。
HPV18 感染在宫颈 NECA 中占主导地位。HPV 基因型的差异可能与 NECA 的临床病理特征和发病途径有关。HPV16 和 HPV18 的疫苗接种可能为近 90%的宫颈 NECA 病例提供保护。
