Department of Craniomaxillofacial and Oral Surgery (Head: Klaus Wilhelm Grätz, MD, DMD, PhD), University of Zurich, Frauenklinikstrasse 24, 8091 Zurich, Switzerland.
J Craniomaxillofac Surg. 2012 Jul;40(5):409-15. doi: 10.1016/j.jcms.2011.07.012. Epub 2011 Aug 26.
The aim of this study was to investigate de novo bone formation following ectopic site implantation of bone substitutes covered by periosteum, with and without the application of autologous platelet-rich plasma (PRP). Twenty-four weeks after subcutaneous implantation of various bone substitutes (bovine hydroxyapatite (bHAP), phycogenic hydroxyapatite (pHAP), and bioglass (BG)) in 35 mini-pigs, bone regeneration rates were compared microradiographically and histologically. Without PRP, bHAP showed a mean de novo bone formation of 32.41%±29.99, in contrast to the other substitute materials where no mineralization could be detected. In combination with PRP, in the bHAP (63.61%±12.98; p±0.03) and pHAP (34.37±29.38; p=0.015) group, significantly higher de novo bone formation was ascertained than without PRP. No ossification could be detected in the BG group. In conclusion, bHAP and pHAP bone substitutes in combination with PRP showed a significant positive effect on periosteal cells by de novo bone formation after ectopic, subcutaneous, low-vascular site implantation.
本研究旨在探讨在骨替代物表面覆盖骨膜，并应用或不应用自体富血小板血浆（PRP）的情况下，异位植入部位的新骨形成情况。在 35 只小型猪的皮下植入不同骨替代物（牛羟磷灰石（bHAP）、藻酸钙羟磷灰石（pHAP）和生物玻璃（BG））24 周后，通过微射线照相术和组织学比较骨再生率。不使用 PRP 时，bHAP 的新生骨形成率为 32.41%±29.99%，而其他替代材料则未检测到矿化。与 PRP 联合应用时，bHAP（63.61%±12.98;p±0.03）和 pHAP（34.37±29.38;p=0.015）组的新生骨形成率明显高于未使用 PRP 的组。BG 组未检测到骨化。综上所述，bHAP 和 pHAP 骨替代物与 PRP 联合应用，在异位、皮下、低血管部位植入后，对骨膜细胞有明显的促新骨形成作用。
