Choi Won-Hyuk, Kim Yong-Deok, Song Jae-Min, Shin Sang-Hun
Department of Oral and maxillofacial surgery, School of dentistry, Pusan National University, Yangsan, 50612, Republic of Korea.
Department of Oral and maxillofacial surgery, Pusan National University Hospital, Pusan, 49241, Republic of Korea.
Maxillofac Plast Reconstr Surg. 2021 Feb 10;43(1):5. doi: 10.1186/s40902-021-00290-x.
Stability of the grafted bone volume is one of the important factors to the success of alveolar bone grafts. For this, platelet-rich plasma (PRP) or fibrin sealant is mixed with the bone graft material. Bio-Oss® is a protein-free bovine mineral commonly used in bone graft procedures. The grafting particles are commonly combined with a standard fibrin sealant (Tisseel®) to fabricate a plastic implantable product. The purpose of this experiment was to evaluate the efficacy of fibrin sealant (Tisseel®) in bone regeneration performance in a rabbit maxillary sinus model.
A total of five 3.5 kg weight New Zealand white rabbits were used for the study. After elevating the sinus membrane in both maxillary sinus cavities, Bio-Oss® mixed with normal saline (group 1) was filled into the right side, and Tisseel® mixed Bio-Oss® (group 2) was inserted into the other side. The bone mineral density and bone volume were analyzed with microscopic computed tomography (micro-CT) and histomorphometric 12 weeks after application.
Histologically, new bone formation rate was 14.8%, and grafted bone rate was 70.5% in group 1. In group 2, they were 18.5% and 60.4%, respectively. According to micro-CT analysis, bone mineral density (mg/cm, BMD) was 2.5% larger in group 1.
The findings from this study suggest that, although the difference in the bone formation between group 1 and group 2 appears to be insignificant, group 2 had an advantage in using smaller amount of bone substances to achieve the reliable bone formation.
移植骨体积的稳定性是牙槽骨移植成功的重要因素之一。为此，富血小板血浆（PRP）或纤维蛋白封闭剂会与骨移植材料混合使用。Bio-Oss®是一种常用于骨移植手术的无蛋白牛骨矿物质。移植颗粒通常与标准纤维蛋白封闭剂（Tisseel®）结合，制成可植入的塑料制品。本实验的目的是评估纤维蛋白封闭剂（Tisseel®）在兔上颌窦模型中骨再生性能的疗效。
本研究共使用了5只体重3.5千克的新西兰白兔。在上颌窦双侧掀起窦膜后，右侧填充与生理盐水混合的Bio-Oss®（第1组），另一侧植入与Tisseel®混合的Bio-Oss®（第2组）。应用12周后，通过显微计算机断层扫描（micro-CT）和组织形态计量学分析骨矿物质密度和骨体积。
组织学上，第1组新骨形成率为14.8%，移植骨率为70.5%。在第2组中，它们分别为18.5%和60.4%。根据micro-CT分析，第1组骨矿物质密度（mg/cm，BMD）高2.5%。
本研究结果表明，虽然第1组和第2组之间的骨形成差异似乎不显著，但第2组在使用较少量骨物质实现可靠骨形成方面具有优势。
