Institute of Chemical Engineering, Research Group Environmental Microbiology and Molecular Ecology, Vienna University of Technology, Gumpendorferstraße 1A/166-5-2, A-1060 Vienna, Austria.
J Microbiol Methods. 2011 Nov;87(2):189-94. doi: 10.1016/j.mimet.2011.08.006. Epub 2011 Aug 18.
Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accomplished before. For this purpose, a species specific PCR method was selected based on the results received for type strains and genotypically characterised environmental strains. For the comparative investigation type strains as well as presumptive C. perfringens isolates from water and faeces samples were used. The acid phosphatase test revealed higher percentage (92%) of correctly identified environmental strains (n=127) than the ISO_LGMN procedure (83%) and proved to be a sensitive and reliable confirmation method.
产气荚膜梭菌常被用作粪便污染的指示菌，以及监测水处理工艺的效果。出于这些目的，区分产气荚膜梭菌和其他梭菌至关重要，这通常通过 ISO/CD 6461-2:2002（ISO_LGMN：乳糖发酵、明胶液化、运动性和硝酸盐还原）中提出的表型标准试验来完成。由于 ISO_LGMN 程序耗时且劳动强度大，因此研究了酸性磷酸酶试验是否可以作为确认的一种更快速的替代方法。我们的研究目的是使用经基因分型鉴定的菌株评估和比较这两种表型方法的确认结果，据我们所知，这在以前尚未完成。为此，基于对典型菌株和经基因分型鉴定的环境菌株的研究结果，选择了一种基于物种特异性的 PCR 方法。为了进行比较研究，使用了典型菌株和来自水和粪便样本的推定产气荚膜梭菌分离株。酸性磷酸酶试验显示，经鉴定的环境菌株（n=127）的正确识别率（92%）高于 ISO_LGMN 程序（83%），并被证明是一种敏感且可靠的确认方法。
