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比较 mCP 和 TSC 培养基以对地表水样本进行计数。

Comparison of mCP and TSC Media to Enumerate in Surface Water Samples.

机构信息

Department of Environmental Health and Safety National Institute of Public Health National Institute of Hygiene - National Research Institute, Warsaw, Poland.

出版信息

Pol J Microbiol. 2023 Dec 16;72(4):413-419. doi: 10.33073/pjm-2023-039. eCollection 2023 Dec 1.

DOI:10.33073/pjm-2023-039
PMID:37999986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10725159/
Abstract

The bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media. The results showed that, although the procedure using the mCP medium was more sensitive and specific, higher recoveries were obtained in the tests based on the TSC medium.

摘要

这些细菌被用来评估水质作为指示参数。如果检测到,它可以确认过去粪便污染的发生。在水样中进行的测试通常通过膜过滤来完成,其中过滤器在厌氧条件下在选择性培养基上孵育。可用的培养基包括 mCP 和 TSC。本研究的目的是比较从地表水样品中回收(包括孢子)的相对回收率,并确定使用这两种培养基的膜过滤方法的性能特征。结果表明,尽管使用 mCP 培养基的程序更敏感和更具特异性,但基于 TSC 培养基的测试中获得了更高的回收率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbf2/10725159/98d2de997424/j_pjm-2023-039_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbf2/10725159/dfd824b8baf0/j_pjm-2023-039_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbf2/10725159/98d2de997424/j_pjm-2023-039_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbf2/10725159/dfd824b8baf0/j_pjm-2023-039_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbf2/10725159/98d2de997424/j_pjm-2023-039_fig_002.jpg

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本文引用的文献

1
Use of bacterial spores in monitoring water quality and treatment.细菌孢子在水质监测与处理中的应用。
J Water Health. 2018 Aug;16(4):491-500. doi: 10.2166/wh.2018.013.
2
Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water.酸磷酸酶试验证明优于从水中分离的产气荚膜梭菌菌株的标准表型鉴定程序。
J Microbiol Methods. 2011 Nov;87(2):189-94. doi: 10.1016/j.mimet.2011.08.006. Epub 2011 Aug 18.
3
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
评估酸性磷酸酶作为从水中分离出的产气荚膜梭菌的确证试验。
Lett Appl Microbiol. 2006 Apr;42(4):418-24. doi: 10.1111/j.1472-765X.2006.01867.x.
4
Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection.再生水中病原体减少及公共卫生防护指标生物范式的有效性。
Appl Environ Microbiol. 2005 Jun;71(6):3163-70. doi: 10.1128/AEM.71.6.3163-3170.2005.
5
Enumeration of Clostridium perfringens spores in groundwater samples: comparison of six culture media.地下水样中产气荚膜梭菌孢子的计数:六种培养基的比较
J Microbiol Methods. 2004 May;57(2):175-80. doi: 10.1016/j.mimet.2003.12.010.
6
Evaluation of different methods for the detection of Clostridium perfringens phosphatases.检测产气荚膜梭菌磷酸酶的不同方法评估
Int J Food Microbiol. 2003 Jan 26;82(1):81-6. doi: 10.1016/s0168-1605(02)00244-1.
7
Evaluation of fluorogenic TSC agar for recovering Clostridium perfringens in groundwater samples.用于从地下水样本中回收产气荚膜梭菌的荧光TSC琼脂的评估。
Water Sci Technol. 2001;43(12):201-4.
8
Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water.用于水中产气荚膜梭菌膜过滤计数的两种培养基的评估。
Lett Appl Microbiol. 1998 Dec;27(6):323-7. doi: 10.1046/j.1472-765x.1998.00454.x.
9
Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts.产气荚膜梭菌和体细胞性大肠杆菌噬菌体作为饮用水处理对病毒和原生动物囊肿处理效率的指标。
Appl Environ Microbiol. 1993 Aug;59(8):2418-24. doi: 10.1128/aem.59.8.2418-2424.1993.
10
Membrane filter enumeration method for Clostridium perfringens.产气荚膜梭菌的膜过滤计数法。
Appl Environ Microbiol. 1979 Jan;37(1):55-66. doi: 10.1128/aem.37.1.55-66.1979.