Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Forensic Sci Int Genet. 2012 May;6(3):413-8. doi: 10.1016/j.fsigen.2011.08.003. Epub 2011 Aug 27.
Fourteen Y-STR loci (DYS458, DYS439, Y-GATA H4, DYS576, DYS447, DYS460, DYS456, YGATA A10, DYS437, DYS449, DYS570, DYS635 or Y-GATA C4, DYS448 and DYS438) were analysed in 873 males from eight northern Brazil populations: Belém (N=400), Santarém (N=69), Manaus (N=75), Macapá (N=65), Palmas (N=30), Rio Branco (N=32), Porto Velho (N=135) and Boa Vista (N=67). A total of 871 different haplotypes were identified, of which 869 were unique. The panel's estimated total haplotype diversity (HD) is 0.9988, and its discrimination capacity (DC) is 0.9980. The lowest estimates of genetic diversity correspond to markers Y-GATA H4 (0.550) and DYS460 (0.581), and the greatest (above 0.700) to markers DYS458, DYS576, DYS447, YS449, DYS570 and DYS635. The genetic parameters obtained were higher for the 14-Y-STR panel than that for the minimum haplotype set (HD=0.9969; DC=0.76) and the parameters were similar to those obtained with the panel of 17 YSTR of YHRD (HD=0.9987; DC=0. 9870). The analysis of molecular variance (AMOVA) indicated that most of the genetic variance is found within populations and a smaller, but significant part, is found among populations (R(ST)=0.027, p value=0.009). The data when compared with those from African, Amerindian and European populations have shown no significant genetic distance between northern Brazil populations and Europeans, but there is a significant genetic distance when compared to Africans and Amerindians. The discrimination capacity of the markers shows a high potential for forensic analysis.
在来自巴西北部 8 个地区的 873 名男性中分析了 14 个 Y-STR 基因座（DYS458、DYS439、Y-GATA H4、DYS576、DYS447、DYS460、DYS456、YGATA A10、DYS437、DYS449、DYS570、DYS635 或 Y-GATA C4、DYS448 和 DYS438）：贝伦（N=400）、圣塔伦（N=69）、马瑙斯（N=75）、马卡帕（N=65）、帕尔马斯（N=30）、里奥布兰科（N=32）、波多韦柳（N=135）和博阿维斯塔（N=67）。确定了 871 种不同的单倍型，其中 869 种是独特的。该面板的总估计单倍型多样性（HD）为 0.9988，其鉴别能力（DC）为 0.9980。遗传多样性最低的标记是 Y-GATA H4（0.550）和 DYS460（0.581），最高的标记超过 0.700，包括 DYS458、DYS576、DYS447、YS449、DYS570 和 DYS635。与最小单倍型组（HD=0.9969；DC=0.76）相比，14 个 Y-STR 面板获得的遗传参数更高，与 YHRD 的 17 个 YSTR 面板获得的参数相似（HD=0.9987；DC=0.9870）。分子方差分析（AMOVA）表明，遗传方差主要存在于群体内部，而群体间的遗传方差较小，但具有统计学意义（R(ST)=0.027，p 值=0.009）。与非洲、美洲印第安人和欧洲人群的数据相比，巴西北部人群与欧洲人群之间没有显著的遗传距离，但与非洲和美洲印第安人群之间存在显著的遗传距离。标记的鉴别能力显示出在法医分析方面有很高的潜力。
