Corpeleijn Willemijn E, van Elburg Ruurd M, Kema Ido P, van Goudoever Johannes B
Division of Neonatology, Erasmus MC - Sophia Children's Hospital, Rotterdam, The Netherlands.
Methods Mol Biol. 2011;763:95-104. doi: 10.1007/978-1-61779-191-8_6.
Infants born prematurely have an enhanced intestinal permeability compared to healthy term infants. This enhanced permeability might be a contributing factor in the development of Necrotising Enterocolitis. The assessment of intestinal permeability in premature neonates with sugar absorption tests has been proven to be safe and of minimal burden to the infant. After enteral administration of a test solution containing lactulose and mannitol, the excretion of these sugars is measured in urine, and the ratio is calculated. The lactulose and mannitol concentrations in urine can be measured by the use of a gas chromatograph after pre-purification and derivatisation of the sample. Non-invasive assessment of intestinal permeability can be useful in monitoring the effects of experimental (nutritional) therapy.
与健康足月儿相比，早产儿的肠道通透性增强。这种增强的通透性可能是坏死性小肠结肠炎发病的一个促成因素。已证实，通过糖吸收试验评估早产儿的肠道通透性是安全的，且对婴儿的负担极小。经肠道给予含有乳果糖和甘露醇的测试溶液后，测量尿液中这些糖的排泄量，并计算其比率。在对样品进行预纯化和衍生化处理后，可使用气相色谱仪测量尿液中的乳果糖和甘露醇浓度。肠道通透性的非侵入性评估有助于监测实验性（营养）疗法的效果。
