Schneider Jürgen E
British Heart Foundation Experimental MR Unit (BMRU), Department of Cardiovascular Medicine, University of Oxford, Headington, Oxford, OX3 9DU, UK.
Methods Mol Biol. 2011;771:387-405. doi: 10.1007/978-1-61779-219-9_20.
High-resolution magnetic resonance cine imaging (cine-MRI) allows for a non-invasive assessment of ventricular function and mass in normal mice and in genetically and surgically modified mouse models of cardiac disease. The assessment of myocardial mass and function by cine-MRI does not rely on geometric assumptions, as the hearts are covered from the base to the apex, typically by a stack of two-dimensional images. The MR data acquisition is then followed by image segmentation of specific cine frames in each slice to obtain geometric and functional parameters, such as end-diastolic volume (EDV), end-systolic volume (ESV) or ejection fraction (EF). This technique has been well established in clinical routine application and it is now also becoming the reference method in experimental cardiovascular MRI. The cine images are typically acquired in short- and long-axis orientations of the heart to facilitate an accurate assessment of cardiac functional parameters. These views can be difficult to identify, particularly in animals with diseased hearts. Furthermore, data analysis can be the source of a systematic error, mainly for myocardial mass measurement. We have established protocols that allow for a quick and reproducible way of obtaining the relevant cardiac views for cine-MRI, and for accurate image analysis.
高分辨率磁共振电影成像（cine-MRI）能够对正常小鼠以及心脏疾病的基因改造和手术改造小鼠模型的心室功能和质量进行无创评估。通过cine-MRI评估心肌质量和功能不依赖于几何假设，因为心脏从心底到心尖通常由一系列二维图像覆盖。然后在采集MR数据之后，对每个切片中的特定电影帧进行图像分割，以获得几何和功能参数，如舒张末期容积（EDV）、收缩末期容积（ESV）或射血分数（EF）。该技术在临床常规应用中已得到充分确立，现在也正成为实验性心血管MRI的参考方法。电影图像通常在心脏的短轴和长轴方向采集，以便于准确评估心脏功能参数。这些视图可能难以识别，尤其是在患有心脏疾病的动物中。此外，数据分析可能是系统误差的来源，主要是在心肌质量测量方面。我们已经建立了方案，能够以快速且可重复的方式获取cine-MRI相关的心脏视图，并进行准确的图像分析。
