Department of Medical Microbiology and Virology, Faculty of Health Science, University of the Free State, Bloemfontein, South Africa.
Epidemiol Infect. 2012 Jun;140(6):1095-101. doi: 10.1017/S0950268811001580. Epub 2011 Aug 30.
There is currently no information regarding the genetic diversity of HPV-6 variants circulating in South Africa. The aim of this study was to determine the HPV-6 variants affecting patients with recurrent respiratory papillomatosis, to determine whether mutations correlate with disease severity and identify molecular determinants of virulence with prognostic relevance. HPV-6 variants were identified based on genome changes within the 712-991 bp region encompassing the non-coding region (URR) of the genome, with variations in length resulting from insertions and duplications, and the 453-bp gene encoding the E6 protein. Based on manual comparison of sequence data from the URR, the isolates were identified as HPV-6a and HPV-6vc variants. Three novel HPV-6 variants were identified: one based on a mutation in the E6 region; two based on changes in the URR including a unique substitution detected in three isolates and an insertion and 170-bp duplication in the URR genome in one patient, who had clinical features of severe disease.
目前尚无关于南非 HPV-6 变异株遗传多样性的信息。本研究旨在确定影响复发性呼吸道乳头状瘤病患者的 HPV-6 变异株，确定突变是否与疾病严重程度相关，并确定具有预后相关性的毒力分子决定因素。HPV-6 变异株的鉴定基于基因组中 712-991bp 区域内的基因组非编码区 (URR) 的基因组变化，由于插入和重复导致长度发生变化，以及编码 E6 蛋白的 453bp 基因。基于 URR 序列数据的手动比较，分离株被鉴定为 HPV-6a 和 HPV-6vc 变异株。鉴定出三种新型 HPV-6 变异株：一种基于 E6 区域的突变；两种基于 URR 的变化，包括在三个分离株中检测到的独特取代和在一个患者的 URR 基因组中发现的插入和 170bp 重复，该患者具有严重疾病的临床特征。
