Dias Marina Carrara, Stuqui Bruna, Provazzi Paola Jocelan Scarin, Bittar Cíntia, Candido Natália Maria, de Matos Renata Prandini Adum, Badial Rodolfo Miglioli, do Bonfim Caroline Measso, Melli Patricia Pereira Dos Santos, Quintana Silvana Maria, Cordeiro José Antônio, Rahal Paula, Calmon Marilia de Freitas
Institute of Biosciences, Letters and Exact Sciences of São Paulo State University, São José do Rio Preto, SP, Brazil.
Clinical Hospital of Faculty of Medicine of Ribeirão Preto, São Paulo University, Ribeirão Preto, SP, Brazil.
Adv Virol. 2019 May 2;2019:5697573. doi: 10.1155/2019/5697573. eCollection 2019.
Condyloma acuminata (CA), or genital warts, are benign proliferative epidermal or mucous lesions that are caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11. HPV variants are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variant lineages have been studied, and there are ongoing attempts to correlate these genetic variants with different clinical findings of infection. Therefore, the aims of this study were to detect variants and nucleotide alterations present in the E6 regions of HPV types 6 and 11 found in CA samples, to correlate the HPV presence with the clinical-pathological data of the patients, and to determine phylogenetic relationships with variants from other places in the world. The E6 regions of 25 HPV6 samples and 7 HPV11 samples from CA were amplified using PCR with specific primers. The products were ligated to a cloning vector and five colonies of each sample were sequenced to observe the nucleotide alterations. Twelve samples were identified as the HPV6B3 variant, presenting the mutation (guanine) G474A (adenine), and one of them also showed the mutation (thymine) T369G. The other 13 patients were positive for HPV6B1 without nucleotide alterations. In the analysis of the HPV11 samples, all patients showed the mutations T137C and (cytosine) C380T. One patient also presented the nucleotide alteration T410C. None of the mutations found in the 32 analyzed samples resulted in amino acid changes. Patient age, local occurrence, and HIV infection did not show significant association with HPV infection. Besides, the data found in this study did not show a relationship with the geographical region of isolation when compared to other data from different regions of the world. In this way, despite the nucleotide alterations found, it was not possible to observe amino acid changes and variants grouping according to geographical region.
尖锐湿疣（CA），即生殖器疣，是由人乳头瘤病毒（HPV）感染引起的良性增生性表皮或黏膜病变，主要由低风险的6型和11型HPV引起。HPV变异体被定义为L1基因核苷酸序列中同一性大于98%的病毒序列。基于这一标准，对HPV6和11变异谱系进行了研究，并且正在尝试将这些基因变异与感染的不同临床发现相关联。因此，本研究的目的是检测CA样本中HPV6型和11型E6区域存在的变异体和核苷酸改变，将HPV的存在情况与患者的临床病理数据相关联，并确定与世界其他地区变异体的系统发育关系。使用特异性引物通过PCR扩增来自CA的25个HPV6样本和7个HPV11样本的E6区域。将产物连接到克隆载体上，对每个样本的五个菌落进行测序以观察核苷酸改变。12个样本被鉴定为HPV6B3变异体，存在突变（鸟嘌呤）G474A（腺嘌呤），其中一个样本还显示出突变（胸腺嘧啶）T369G。其他13名患者HPV6B1呈阳性，无核苷酸改变。在对HPV11样本的分析中，所有患者均显示出突变T137C和（胞嘧啶）C380T。一名患者还出现了核苷酸改变T410C。在32个分析样本中发现的突变均未导致氨基酸变化。患者年龄、局部发病情况和HIV感染与HPV感染均无显著关联。此外，与世界不同地区的其他数据相比，本研究中发现的数据与分离的地理区域无关。这样，尽管发现了核苷酸改变，但无法观察到氨基酸变化以及根据地理区域进行的变异体分组。
