García-Romero Carmen S, Akaki-Caballero Matsuharu, Saavedra-Mendoza Ana G, Guzmán-Romero Ana K, Canto Patricia, Coral-Vázquez Ramón M
Laboratorio de Biología Molecular y Virología, Instituto Nacional de Perinatología, Secretaría de Salud, México, D.F., Mexico.
Servicio de Otorrinolaringología, Centro Médico Nacional "20 de Noviembre", Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., Mexico.
Auris Nasus Larynx. 2015 Oct;42(5):385-9. doi: 10.1016/j.anl.2015.03.006. Epub 2015 Apr 20.
This study aimed to identify the isotype of human papillomavirus (HPV) in fresh tissue samples of 35 male adults with adult recurrent adult respiratory papillomatosis which may be important to define the precise etiology of the disease, and determine the therapeutic and prophylactic measures.
A total of 35 adult male patients diagnosed with active RRP who have been treated for several years were included in the study. DNA of patients was extracted from fresh biological samples and analyzed by PCR and a Linear Array® HPV Genotyping system.
Most cases (95%) corresponded to adult-onset of RRP. A questionnaire was applied to obtain demographic and clinical data. Using a PCR-based detection system all patients showed the presence of HPV; 80% were positive for HPV-6, 8% for HPV-11 and one for HPV-16.
Most patients presented HPV-6 and consequently it was not feasible to correlate clinical and demographic characteristics with viral type. Besides, co-infections were not evident. This knowledge may be relevant to delineate therapeutic and preventive measures.
本研究旨在确定35例患有成人复发性呼吸道乳头状瘤病的成年男性新鲜组织样本中的人乳头瘤病毒（HPV）亚型，这对于明确该疾病的确切病因以及确定治疗和预防措施可能具有重要意义。
本研究纳入了35例被诊断为活动性复发性呼吸道乳头状瘤病且已接受数年治疗的成年男性患者。从新鲜生物样本中提取患者的DNA，并通过聚合酶链反应（PCR）和线性阵列HPV基因分型系统进行分析。
大多数病例（95%）符合成人复发性呼吸道乳头状瘤病的成人发病情况。应用问卷调查以获取人口统计学和临床数据。使用基于PCR的检测系统，所有患者均显示存在HPV；80%为HPV-6阳性，8%为HPV-11阳性，1例为HPV-16阳性。
大多数患者表现为HPV-6感染，因此将临床和人口统计学特征与病毒类型相关联是不可行的。此外，未发现明显的合并感染情况。这些知识可能与制定治疗和预防措施相关。
