利用重组糖苷转移酶和内切糖苷神经酰胺合成酶进行糖脂合成。
Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase.
机构信息
Department of Chemistry and Centre for High-Throughput Biology, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, Canada V6T 1Z1.
出版信息
Chem Commun (Camb). 2011 Oct 14;47(38):10806-8. doi: 10.1039/c1cc13885e. Epub 2011 Aug 30.
PMID:21879043
Abstract
Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.
摘要
节旋光菌内切糖基神经酰胺酶 II 的糖基合成酶突变体能够有效地合成复杂糖脂。糖基氟化物供体可以通过乳酰基氟化物的顺序糖基转移酶催化糖基化来组装。或者，乳酰基氟化物可以在随后的糖基化步骤之前与神经酰胺连接。
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