Center for Infection and Immunity, Columbia University, 722 West 168th Street, New York, NY 10032, USA.
J Virol. 2011 Nov;85(21):11520-5. doi: 10.1128/JVI.05317-11. Epub 2011 Aug 31.
Many of our fatal "civilization" infectious diseases have arisen from domesticated animals. Although picornaviruses infect most mammals, infection of a companion animal is not known. Here we describe the identification and genomic characterization of the first canine picornavirus. Canine kobuvirus (CKoV), identified in stool samples from dogs with diarrhea, has a genomic organization typical of a picornavirus and encodes a 2,469-amino-acid polyprotein flanked by 5' and 3' untranslated regions. Comparative phylogenetic analysis using various structural and nonstructural proteins of CKoV confirmed it as the animal virus homolog most closely related to human Aichivirus (AiV). Bayesian Markov chain Monte Carlo analysis suggests a mean recent divergence time of CKoV and AiV within the past 20 to 50 years, well after the domestication of canines. The discovery of CKoV provides new insights into the origin and evolution of AiV and the species specificity and pathogenesis of kobuviruses.
我们许多致命的“文明”传染病都源自驯化动物。虽然小核糖核酸病毒感染大多数哺乳动物，但伴侣动物的感染情况尚不清楚。在这里，我们描述了首例犬小核糖核酸病毒的鉴定和基因组特征。从患有腹泻的犬的粪便样本中鉴定出的犬科柯萨奇病毒（CKoV）具有小核糖核酸病毒的典型基因组组织，编码一个由 5'和 3'非翻译区侧翼的 2469 个氨基酸多蛋白。使用 CKoV 的各种结构和非结构蛋白进行的比较系统发育分析证实，它是与人类艾柯病毒（AiV）最密切相关的动物病毒同源物。贝叶斯马尔可夫链蒙特卡罗分析表明，CKoV 和 AiV 的最近平均分歧时间在过去 20 到 50 年之间，远在犬类驯化之后。CKoV 的发现为 AiV 的起源和进化以及柯萨奇病毒的物种特异性和发病机制提供了新的见解。
