Shanghai Center of Agri-Products Quality and Safety, Shanghai, 201400, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, Anhui, China.
Arch Virol. 2023 Mar 14;168(4):112. doi: 10.1007/s00705-023-05710-z.
In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, the coinfection rates of canine distemper virus, canine coronavirus, canine astrovirus, canine norovirus, and canine rotavirus were 26.67%, 20.00%, 73.33%, 0%, and 20.00%, respectively. Phylogenetic analysis based on partial VP1 sequences identified a novel canine kobuvirus that was a recombinant of canine and feline kobuvirus. Bayesian evolutionary analysis revealed that the rate of evolution of the VP1 gene of canine kobuvirus was 1.36 × 10 substitutions per site per year (95% highest posterior density interval, 6.28 × 10 - 4.30 × 10 substitutions per site per year). Finally, the divergence time of VP1 was around 19.44 years ago (95% highest posterior density interval, 12.96-27.57 years).
在这项研究中，通过定量实时逆转录聚合酶链反应，对来自狗舍的 60 只流浪狗的直肠样本进行了犬科博卡病毒和其他肠道病毒的筛查。在 60 个样本中，有 25%（15/60）的样本检测到了犬科博卡病毒。在 15 个阳性样本中，犬瘟热病毒、犬冠状病毒、犬星状病毒、犬诺如病毒和犬轮状病毒的混合感染率分别为 26.67%、20.00%、73.33%、0%和 20.00%。基于部分 VP1 序列的系统发育分析鉴定出一种新型的犬科博卡病毒，它是犬科和猫科博卡病毒的重组病毒。贝叶斯进化分析显示，犬科博卡病毒 VP1 基因的进化率为每年每一位点 1.36×10 个取代（95%最高后验密度区间为 6.28×10-4.30×10 个取代/每一位点/年）。最后，VP1 的分歧时间约为 19.44 年前（95%最高后验密度区间为 12.96-27.57 年）。
