Department of Clinical Laboratory Medicine, Wakayama Medical University, 811-1, Kimiidera, 641-8509 Wakayama City, Japan.
J Clin Endocrinol Metab. 2011 Nov;96(11):E1857-61. doi: 10.1210/jc.2011-1180. Epub 2011 Aug 31.
Neuropilin-2 (Nrp2) is a coreceptor for vascular endothelial growth factor-D (VEGF-D) that is expressed on the surface of endothelial cells. Recently, Nrp2 was shown to play a role in lymph node metastasis and promotion of cancer cell migration. VEGF-D also promotes lymphangiogenesis, which in turn promotes tumor metastasis.
The aim was to study the role of neuropilin-2 in lymph node metastasis in human papillary thyroid carcinoma (PTC).
Expression of Nrp2 was studied by immunohistochemistry and the relationship between Nrp2 expression and lymph node metastasis, VEGF-D expression and other established clinicopathological variables were analyzed in PTC. The effects of neutralizing anti-Nrp2 antibody on VEGF-D-induced invasion and migration were assessed in PTC cell lines.
Nrp2 expression was observed in 64.3% (36 of 56) of the PTC patients. Nrp2 expression was significantly correlated with lymph node metastasis (P = 0.0216) and VEGF-D expression (P = 0.0034). VEGF-D was shown to promote filopodia formation and cancer cell migration and invasion by K1 and B-CPAP cells. These responses were significantly blocked by neutralizing anti-Nrp2 antibody.
Nrp2 expression was correlated with lymph node metastasis and VEGF-D expression in PTC. Our data also showed a role for Nrp2 in regulating VEGF-D-induced invasion and migration in vitro.
神经纤毛蛋白-2（Nrp2）是血管内皮生长因子-D（VEGF-D）的核心受体，在血管内皮细胞表面表达。最近，Nrp2 被证明在淋巴结转移和促进癌细胞迁移中发挥作用。VEGF-D 还促进淋巴管生成，进而促进肿瘤转移。
本研究旨在探讨神经纤毛蛋白-2（Nrp2）在人甲状腺乳头状癌（PTC）淋巴结转移中的作用。
通过免疫组织化学研究 Nrp2 的表达，并分析 Nrp2 表达与淋巴结转移、VEGF-D 表达与其他已建立的临床病理变量之间的关系在 PTC 中。在 PTC 细胞系中评估中和抗-Nrp2 抗体对 VEGF-D 诱导的侵袭和迁移的影响。
在 56 例 PTC 患者中，观察到 64.3%（36/56）的患者 Nrp2 表达。Nrp2 表达与淋巴结转移（P=0.0216）和 VEGF-D 表达（P=0.0034）显著相关。VEGF-D 被证明通过 K1 和 B-CPAP 细胞促进伪足形成和癌细胞迁移和侵袭。这些反应被中和抗-Nrp2 抗体显著阻断。
Nrp2 表达与 PTC 中的淋巴结转移和 VEGF-D 表达相关。我们的数据还表明 Nrp2 在调节 VEGF-D 诱导的体外侵袭和迁移中起作用。
