IVF Nagata Clinic, Fukuoka, 810-0001, Japan.
J Assist Reprod Genet. 2011 Nov;28(11):1099-104. doi: 10.1007/s10815-011-9634-5. Epub 2011 Sep 1.
To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI).
Zygotes that had cleaved to two-cell stage by 27 h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups.
Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8 μm(2)), lengths (14.7 vs. 13.4 μm) and PolScope retardance were also significantly greater in the early cleaving group.
Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential.
研究人卵母细胞减数分裂纺锤体特征与卵胞浆内单精子注射（ICSI）后第一次卵裂的时间之间的关系。
将 ICSI 后 27 小时已分裂至两细胞阶段的受精卵分为早裂组，其余的为晚裂组。比较两组 PolScope 先前成像的减数分裂纺锤体参数。
在 384 个胚胎中，163 个被归类为早裂，221 个为晚裂。与晚裂相比，早裂的囊胚形成率或胚胎移植日 2 的妊娠率显著更高（52.4%对 24.4%或 32.6%对 11.4%）。早裂组纺锤体面积（108.0 对 89.8 μm(2)）、长度（14.7 对 13.4 μm）和 PolScope 延迟也显著更大。
减数分裂纺锤体参数决定第一次卵裂的时间，是人类胚胎发育潜能的有力指标。
