Fang Cong, Tang Mandy, Li Tao, Peng Wen-Lin, Zhou Can-Quan, Zhuang Guang-Lun, Leong Milton
Reproductive Medical Center, The First Affiliated Hospital of SUN Yat-sen University, No. 58 Rd. zhongshan er, Guangzhou, China.
J Assist Reprod Genet. 2007 Nov;24(11):547-51. doi: 10.1007/s10815-007-9171-4. Epub 2007 Sep 27.
The aim of this study is to investigate the relationship between spindle location and embryonic development of in vivo and in vitro matured human oocytes.
The spindles of 134 in vivo matured, 105 in vitro matured oocytes were examined by Polscope at the time of ICSI.
The spindles were visualized in 83.6 and 77.1% of in vivo and in vitro matured oocytes respectively. The rate of fertilization of in vivo matured oocytes with spindles beneath or adjacent to the first polar body (angle of 0-5 degrees) was significantly higher (93.3%) than all other groups. The proportions of various spindle positions did not differ statistically in in vivo and in vitro matured oocytes.
Meiotic spindle location with regard to the first polar body appears to influence fertilization rate.
本研究旨在探讨体内和体外成熟的人卵母细胞纺锤体位置与胚胎发育之间的关系。
在卵胞浆内单精子注射（ICSI）时，通过偏振光显微镜检查134个体内成熟和105个体外成熟卵母细胞的纺锤体。
体内成熟和体外成熟卵母细胞的纺锤体可视化率分别为83.6%和77.1%。纺锤体位于第一极体下方或与其相邻（角度为0 - 5度）的体内成熟卵母细胞的受精率显著更高（93.3%），高于所有其他组。体内和体外成熟卵母细胞中各种纺锤体位置的比例在统计学上没有差异。
减数分裂纺锤体相对于第一极体的位置似乎会影响受精率。
