Tomari Hiroyuki, Honjo Ko, Kunitake Katsuko, Aramaki Natsumi, Kuhara Saori, Hidaka Naomi, Nishimura Kayoko, Nagata Yumi, Horiuchi Toshitaka
IVF Nagata Clinic Fukuoka Japan.
Graduate School of Comprehensive Scientific Research Prefectural University of Hiroshima Hiroshima Japan.
Reprod Med Biol. 2018 Apr 10;17(3):268-274. doi: 10.1002/rmb2.12100. eCollection 2018 Jul.
To investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI).
Analyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40 years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI. The oocytes were classified into three groups, according to spindle size: group A (<90 μm), group B (90-120 μm), and group C (>120 μm).
Overall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups.
The oocytes with a spindle size of 90-120 μm showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically.
探讨人类中期II卵母细胞减数分裂纺锤体大小与卵胞浆内单精子注射（ICSI）后胚胎发育潜能之间的关系。
分析了281例年龄在40岁以下接受ICSI周期治疗患者的1302枚可见减数分裂纺锤体的卵母细胞。在ICSI前使用偏振光显微镜对减数分裂纺锤体进行成像。根据纺锤体大小将卵母细胞分为三组：A组（<90μm）、B组（90 - 120μm）和C组（>120μm）。
总体而言，389枚（29.9%）卵母细胞被分为A组，662枚（50.8%）被分为B组，251枚（19.3%）被分为C组。B组卵母细胞的受精率显著高于A组和C组卵母细胞。B组的囊胚形成率显著高于A组。此外，B组的妊娠率显著高于其他两组。
纺锤体大小为90 - 120μm的卵母细胞比纺锤体较大或较小的卵母细胞显示出更高的受精率、囊胚形成率和临床妊娠率。因此，减数分裂纺锤体大小的测量对临床上识别人类胚胎发育潜能具有积极的预测价值。
