Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India.
J Econ Entomol. 2011 Aug;104(4):1406-14. doi: 10.1603/ec11112.
The majority of plant viruses are dependent on arthropod vectors for spread between plants. Wheat streak mosaic virus (family Potyviridae, genus Tritimovirus, WSMV) is transmitted by the wheat curl mite, Aceria tosichella Keifer, and this virus and vector cause extensive yield losses in most major wheat (Triticum aestivum L.)-growing regions of the world. Many cultivars in use are susceptible to this vector-virus complex, and yield losses of 10-99% have been documented. wheat curl mite resistance genes have been identified in goat grass, Aegilops tauschii (Coss) Schmal., and transferred to hexaploid wheat, but very few varieties contain effectively wheat curl mite resistance, due to virulent wheat curl mite populations. However, wheat curl mite resistance remains an effective strategy to reduce losses due to WSMV. The goal of our project was to identify the most effective, reproducible, and rapid method for assessing wheat curl mite resistance. We also wanted to determine whether mite resistance is affected by WSMV infection, because the pathogen and pest commonly occur together. Single and group wheat curl mite infestations produced similar amounts of leaf rolling and folding on wheat curl mite-susceptible wheat varieties that were independent of initial wheat curl mite infestation. This finding will allow accurate, efficient, large-scale screening of wheat germplasm for wheat curl mite resistance by infesting plants with sections of wheat leaf tissue containing mixed stages of wheat curl mite. The wheat curl mite-resistant breeding line 'OK05312' displayed antibiosis (reduced wheat curl mite population development). The effect of WSMV infection on wheat curl mite reproduction was genotype-dependent. Mite populations increased on infected wheat curl mite- and WSMV-susceptible plants compared with uninfected plants, but WSMV infection had no significant effect on wheat curl mite populations on resistant plants. OK05312 is a strong source of wheat curl mite resistance for wheat breeding programs.
大多数植物病毒依赖节肢动物载体在植物间传播。小麦线条花叶病毒（Potyviridae 科，Tritimovirus 属，WSMV）由麦卷叶螨（Aceria tosichella Keifer）传播，这种病毒和载体在世界上大多数主要的小麦（Triticum aestivum L.）种植区造成广泛的产量损失。许多使用的品种易受这种载体-病毒复合物的影响，已记录到 10-99%的产量损失。在山羊草（Aegilops tauschii（Coss）Schmal.）中已鉴定出抗麦卷叶螨基因，并已转移到六倍体小麦中，但由于麦卷叶螨种群具有毒性，很少有品种含有有效的抗麦卷叶螨性。然而，抗麦卷叶螨仍然是减少 WSMV 损失的有效策略。我们项目的目标是确定评估麦卷叶螨抗性最有效、可重复和快速的方法。我们还想确定螨抗性是否受 WSMV 感染的影响，因为病原体和害虫通常同时发生。单只和多只麦卷叶螨的侵染在易受麦卷叶螨侵染的小麦品种上产生了相似数量的叶片卷曲和褶皱，而与初始麦卷叶螨侵染无关。这一发现将允许通过侵染含有混合阶段麦卷叶螨的小麦叶片组织部分的植物，对小麦种质进行准确、高效、大规模的麦卷叶螨抗性筛选。抗麦卷叶螨的小麦品系“OK05312”表现出抗生性（减少麦卷叶螨种群发育）。WSMV 感染对麦卷叶螨繁殖的影响取决于基因型。与未感染植物相比，受感染的麦卷叶螨和 WSMV 敏感植物上的螨虫种群增加，但 WSMV 感染对抗性植物上的麦卷叶螨种群没有显著影响。OK05312 是小麦抗麦卷叶螨育种计划的一个强有力的来源。
