University of Wisconsin School of Medicine and Public Health.
Child Dev. 2013 Jan-Feb;84(1):58-75. doi: 10.1111/j.1467-8624.2011.01641.x. Epub 2011 Sep 2.
Fifteen-year-old adolescents (N = 109) in a longitudinal study of child development were recruited to examine differences in DNA methylation in relation to parent reports of adversity during the adolescents' infancy and preschool periods. Microarray technology applied to 28,000 cytosine-guanine dinucleotide sites within DNA derived from buccal epithelial cells showed differential methylation among adolescents whose parents reported high levels of stress during their children's early lives. Maternal stressors in infancy and paternal stressors in the preschool years were most strongly predictive of differential methylation, and the patterning of such epigenetic marks varied by children's gender. To the authors' knowledge, this is the first report of prospective associations between adversities in early childhood and the epigenetic conformation of adolescents' genomic DNA.
15 岁的青少年（N=109）参与了一项儿童发展的纵向研究，旨在研究婴儿期和幼儿期父母报告的逆境与 DNA 甲基化差异之间的关系。应用于取自口腔上皮细胞的 DNA 中 28000 个胞嘧啶-鸟嘌呤二核苷酸位点的微阵列技术显示，父母在孩子早期生活中报告高水平压力的青少年之间存在 DNA 甲基化差异。婴儿期的母亲压力源和幼儿期的父亲压力源最能预测 DNA 甲基化的差异，这种表观遗传标记的模式因儿童的性别而异。据作者所知，这是首次报告儿童早期逆境与青少年基因组 DNA 的表观遗传构象之间存在前瞻性关联。
