Mechanical and Industrial Engineering Department, Northeastern University, Boston, MA 02115, USA.
Biomaterials. 2011 Dec;32(34):8753-70. doi: 10.1016/j.biomaterials.2011.08.028. Epub 2011 Aug 31.
The seeding of a porous scaffold with stem cells is a fundamental step in engineering sizeable tissue constructs that are clinically viable. However, a key problem often encountered is inhomogeneous seeding of the cells particularly when the cells are delivered through the thickness of the scaffold. The objective of this study was to establish the quantitative relationships between the cell seeding efficiency and the initial vacuum pressure in a compact perfusion seeding device that uses the effect of differential pressure induced by vacuum to seed cells on a porous scaffold. A transient CFD solution of the fluid flow in the device was used to optimize the initial vacuum pressure for efficient cell seeding. Results indicate that the optimal initial vacuum pressure for homogenous cell seeding is approximately -20 kPa for the seeding device. This study presents a 3-D computational model that can be employed in designing and optimizing cell seeding techniques and corresponding technology.
将干细胞接种到多孔支架中是工程化可临床应用的大尺寸组织构建体的基本步骤。然而，经常遇到的一个关键问题是细胞接种不均匀，特别是当细胞通过支架厚度输送时。本研究的目的是在使用真空引起的压差效应将细胞接种到多孔支架上的紧凑灌注接种装置中，建立细胞接种效率与初始真空压力之间的定量关系。使用装置内流体流动的瞬态 CFD 解来优化用于有效细胞接种的初始真空压力。结果表明，对于均匀细胞接种，接种装置的最佳初始真空压力约为-20 kPa。本研究提出了一种 3-D 计算模型，可用于设计和优化细胞接种技术和相应技术。
