Hematology and Stem Cell Laboratory-Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90610-000, Brazil ; Material Science Post-graduate Programme, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil.
Hematology and Stem Cell Laboratory-Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90610-000, Brazil.
Biomicrofluidics. 2013 Aug 29;7(4):44130. doi: 10.1063/1.4819747. eCollection 2013.
Bio-electrospraying (BES) is a technique used for the processing of cells and can be applied to tissue engineering. The association of BES with scaffold production techniques has been shown to be an interesting strategy for the production of biomaterials with cells homogeneously distributed in the entire structure. Various studies have evaluated the effects of BES on different cell types. However, until the present moment, no studies have evaluated the impact of BES time on mesenchymal stem cells (MSC). Therefore, the aim of this work was to standardise the different parameters of BES (voltage, flow rate, and distance of the needle from the collecting plate) in relation to cell viability and then to evaluate the impact of BES time in relation to viability, proliferation, DNA damage, maintenance of plasticity and the immunophenotypic profile of MSC. Using 15 kV voltage, 0.46 ml/h flow rate and 4 cm distance, it was possible to form a stable and continuous jet of BES without causing a significant reduction in cell viability. Time periods between 15 and 60 min of BES did not cause alterations of viability, proliferation, plasticity, and immunophenotypic profile of the MSC. Time periods above 30 min of BES resulted in DNA damage; however, the DNA was able to repair itself within five hours. These results indicate that bio-electrospraying is an adequate technique for processing MSC which can be safely applied to tissue engineering and regenerative medicine.
生物电喷射（BES）是一种用于处理细胞的技术，可应用于组织工程。BES 与支架生产技术的结合被证明是一种有趣的策略，可用于生产细胞均匀分布在整个结构中的生物材料。各种研究已经评估了 BES 对不同细胞类型的影响。然而，直到目前为止，还没有研究评估 BES 时间对间充质干细胞（MSC）的影响。因此，这项工作的目的是标准化 BES 的不同参数（电压、流速和针与收集板的距离）与细胞活力的关系，然后评估 BES 时间对细胞活力、增殖、DNA 损伤、保持可塑性和 MSC 免疫表型特征的影响。使用 15kV 电压、0.46ml/h 流速和 4cm 距离，可以形成稳定且连续的 BES 射流，而不会导致细胞活力显著降低。BES 时间在 15 到 60 分钟之间不会导致 MSC 的活力、增殖、可塑性和免疫表型特征发生变化。BES 时间超过 30 分钟会导致 DNA 损伤；然而，DNA 能够在五小时内自我修复。这些结果表明，生物电喷射是一种处理 MSC 的合适技术，可安全应用于组织工程和再生医学。
