Liu S C, Oguntimein B, Hufford C D, Clark A M
Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University 38677.
Antimicrob Agents Chemother. 1990 Apr;34(4):529-33. doi: 10.1128/AAC.34.4.529.
Further examination of the active ethanolic extract of the root bark of Cleistopholis patens by using bioassay-directed fractionation resulted in the isolation of a new alkaloid, 3-methoxysampangine (compound I), together with three known alkaloids, eupolauridine (compound II), liriodenine (compound III), and eupolauridine N-oxide (compound IV). The proposed structure of compound I was based on its physicochemical properties and spectral data. 3-Methoxysampangine exhibited significant antifungal activity against Candida albicans, Aspergillus fumigatus, and Cryptococcus neoformans. This is the first report of the isolation of liriodenine (compound III) from the root bark of C. patens.
通过生物活性导向分离法对闭花木根皮的活性乙醇提取物进行进一步研究，分离得到一种新生物碱3-甲氧基桑攀碱（化合物I），以及三种已知生物碱，即优波劳定（化合物II）、鹅掌楸碱（化合物III）和优波劳定N-氧化物（化合物IV）。化合物I的结构推测基于其理化性质和光谱数据。3-甲氧基桑攀碱对白色念珠菌、烟曲霉和新型隐球菌表现出显著的抗真菌活性。这是首次从闭花木根皮中分离得到鹅掌楸碱（化合物III）的报道。
