Tamási G
Phylaxia Veterinary Biologicals Co., Budapest, Hungary.
Rev Sci Tech. 1995 Mar;14(1):75-9.
Testing under laboratory conditions is undoubtedly useful in assessing the activity of disinfectants. However, such testing must be regarded as no more than a preliminary to field trials. This not only indicates the unreliability of laboratory tests, but also poses the wider problem of how any laboratory evaluation can be correlated with the field requirements. Many attempts have therefore been made to devise tests which are suitable to evaluate the in-use requirements of disinfection (e.g. on farms). The aim of these techniques is to record the end-point of a disinfection procedure on the surfaces in animal houses. In view of the need to standardise testing conditions in the field, the author provides details of the optimum time and place for taking samples under field conditions. Samples for culture media should be taken from the floor when dry. The various techniques (i.e. swabbing, agar cylinder, agar carrier, 'ready-to-use' set) should provide information on the end-point of the disinfection. The desired end-points is approximately one viable bacterium per cm2 of surface area. The technique used should demonstrate whether the disinfection has been effective.
在实验室条件下进行测试无疑有助于评估消毒剂的活性。然而，这种测试必须仅被视为现场试验的前奏。这不仅表明了实验室测试的不可靠性，还引发了一个更广泛的问题，即任何实验室评估如何能与现场要求相关联。因此，人们进行了许多尝试来设计适合评估消毒实际使用要求（如在农场）的测试。这些技术的目的是记录动物房舍表面消毒程序的终点。鉴于在现场需要标准化测试条件，作者提供了在现场条件下采集样本的最佳时间和地点的详细信息。用于培养基的样本应在地面干燥时采集。各种技术（即擦拭、琼脂圆柱体、琼脂载体、“即用型”套装）应能提供有关消毒终点的信息。理想的终点是每平方厘米表面积约有一个活细菌。所使用的技术应能证明消毒是否有效。
