Department of Experimental Medical Science, Lund University, Lund, Sweden.
Mol Nutr Food Res. 2011 Sep;55 Suppl 2:S290-3. doi: 10.1002/mnfr.201100231. Epub 2011 Sep 2.
The effects of alkylresorcinols (ARs) isolated from rye bran on adipocyte lipolysis, hormone-sensitive lipase activity and phosphorylation and on phosphorylation of protein kinase A substrates were studied. Preincubation with ARs for 18 h suppressed catecholamine-stimulated lipolysis in 3T3-L1 adipocytes. Furthermore, phosphorylation of hormone-sensitive lipase (HSL), a key lipase responsible for stimulated lipolysis, and phosphorylation of protein kinase A substrates, were diminished after preincubation with ARs, whereas HSL protein expression was unaltered. ARs were also shown to inhibit HSL activity in an in vitro assay.
研究了从小麦麸中分离出的烷基间苯二酚（ARs）对脂肪细胞脂解作用、激素敏感性脂肪酶（HSL）活性和磷酸化以及蛋白激酶 A 底物磷酸化的影响。用 ARs 预孵育 18 小时可抑制 3T3-L1 脂肪细胞中儿茶酚胺刺激的脂解作用。此外，用 ARs 预孵育后，HSL （一种负责刺激脂解的关键脂肪酶）的磷酸化和蛋白激酶 A 底物的磷酸化减少，而 HSL 蛋白表达不变。ARs 还在体外测定中抑制 HSL 活性。
