Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas, Isla de la Cartuja (cicCartuja), Universidad de Sevilla-CSIC, Avda. Américo Vespucio 49, Sevilla 41092, Spain.
FEBS Lett. 2012 Mar 9;586(5):646-52. doi: 10.1016/j.febslet.2011.08.035. Epub 2011 Aug 30.
Transient complexes, with a lifetime ranging between microseconds and seconds, are essential for biochemical reactions requiring a fast turnover. That is the case of the interactions between proteins engaged in electron transfer reactions, which are involved in relevant physiological processes such as respiration and photosynthesis. In the latter, the copper protein plastocyanin acts as a soluble carrier transferring electrons between the two membrane-embedded complexes cytochrome b(6)f and photosystem I. Here we review the combination of experimental efforts in the literature to unveil the functional and structural features of the complex between cytochrome f and plastocyanin, which have widely been used as a suitable model for analyzing transient redox interactions.
瞬变复合物的寿命在微秒到秒之间，对于需要快速转化的生化反应至关重要。这就是参与电子转移反应的蛋白质之间相互作用的情况，这些反应涉及到相关的生理过程，如呼吸和光合作用。在后一种情况下，铜蛋白质体蓝素作为一种可溶性载体，在两个膜嵌入的复合物细胞色素 b(6)f 和光系统 I 之间传递电子。在这里，我们综述了文献中实验研究的结果，揭示了细胞色素 f 和质体蓝素之间复合物的功能和结构特征，该复合物被广泛用作分析瞬态氧化还原相互作用的合适模型。
