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一种改良的用于流式细胞术和皮肤伤口白细胞功能分析的细胞分离方法。

An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes.

机构信息

The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA.

出版信息

J Immunol Methods. 2011 Oct 28;373(1-2):161-6. doi: 10.1016/j.jim.2011.08.013. Epub 2011 Aug 25.

DOI:10.1016/j.jim.2011.08.013
PMID:21889511
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3195830/
Abstract

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

摘要

从全层切除创伤口中分离白细胞已被证明是一个困难的过程，导致细胞产量低，并且对功能测定有很大的限制。鉴于人们对伤口来源细胞群体的分离、鉴定和功能测量的兴趣增加，在此我们描述了一种方法来制备具有改善产量的伤口细胞悬浮液，从而能够进行表型和功能评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/a8f99ddfcd73/nihms321467f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/9f5652852270/nihms321467f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/446746fc0399/nihms321467f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/a8f99ddfcd73/nihms321467f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/9f5652852270/nihms321467f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/446746fc0399/nihms321467f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03cc/3195830/a8f99ddfcd73/nihms321467f3.jpg

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Prevention of NKT cell activation accelerates cutaneous wound closure and alters local inflammatory signals.预防 NKT 细胞激活可加速皮肤伤口闭合，并改变局部炎症信号。
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