Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea.
Biotechnol Adv. 2012 Sep-Oct;30(5):989-1000. doi: 10.1016/j.biotechadv.2011.08.015. Epub 2011 Aug 25.
The increasing oil price and environmental concerns caused by the use of fossil fuel have renewed our interest in utilizing biomass as a sustainable resource for the production of biofuel. It is however essential to develop high performance microbes that are capable of producing biofuels with very high efficiency in order to compete with the fossil fuel. Recently, the strategies for developing microbial strains by systems metabolic engineering, which can be considered as metabolic engineering integrated with systems biology and synthetic biology, have been developed. Systems metabolic engineering allows successful development of microbes that are capable of producing several different biofuels including bioethanol, biobutanol, alkane, and biodiesel, and even hydrogen. In this review, the approaches employed to develop efficient biofuel producers by metabolic engineering and systems metabolic engineering approaches are reviewed with relevant example cases. It is expected that systems metabolic engineering will be employed as an essential strategy for the development of microbial strains for industrial applications.
不断上涨的油价以及对化石燃料使用所带来的环境问题，使我们重新关注利用生物质作为生物燃料生产的可持续资源。然而，开发能够非常高效地生产生物燃料的高性能微生物是至关重要的，这是为了与化石燃料竞争。最近，通过系统代谢工程来开发微生物菌株的策略已经被开发出来，它可以被认为是将系统生物学和合成生物学与代谢工程相结合。系统代谢工程允许成功开发出能够生产多种不同生物燃料的微生物，包括生物乙醇、生物丁醇、烷烃和生物柴油，甚至氢气。在这篇综述中，我们回顾了通过代谢工程和系统代谢工程方法开发高效生物燃料生产菌的方法，并提供了相关的实例。预计系统代谢工程将作为用于工业应用的微生物菌株开发的重要策略。
