Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.
Theriogenology. 2011 Dec;76(9):1744-55. doi: 10.1016/j.theriogenology.2011.07.010. Epub 2011 Sep 3.
The present study compared the changes in isoflavone (daidzein and genistein) and their metabolite (equol and para-ethyl-phenol) concentrations in the blood plasma of cows with induced mastitis and metritis after feeding with soy bean. Sixteen cows were divided into four groups: control for mastitis group, cows with induced mastitis group, control for metritis group, and cows with induced metritis group. All cows were fed a single dose of 2.5 kg of soy bean and then blood samples were taken from the jugular vein for 8 h at predetermined intervals. The concentrations of soy bean-derived isoflavones and their active metabolites were measured in the blood plasma on HPLC system. β-Glucuronidase activity in the blood plasma of cows was measured by fluorometric method. In the blood plasma of cows with induced mastitis and metritis, we found considerably higher concentrations and time-dependent increase in isoflavone metabolites (equol and para-ethyl-phenol) with reference to cyclic cows (P < 0.05). Moreover, we noticed significant decrease of genistein in the blood plasma of the cows with induced metritis compared with control cows (P < 0.05). In addition, in the blood plasma of the cows with induced metritis, we found an increase in β-glucuronidase activity compared with control cows (P < 0.05). In conclusion, health status of the females influenced the concentrations of isoflavone metabolites in the blood plasma of the cows. Experimentally induced mastitis and metritis increased isoflavone absorption, biotransformation and metabolism. Therefore, we suggest that cows with induced mastitis and metritis are more exposed to active isoflavone metabolite actions than healthy cows.
本研究比较了饲喂大豆后患有诱导性乳腺炎和子宫内膜炎的奶牛血液血浆中异黄酮（大豆苷元和染料木黄酮）及其代谢物（黄豆苷元和对乙基苯酚）浓度的变化。将 16 头奶牛分为 4 组：乳腺炎对照 1 组、乳腺炎诱导组、子宫内膜炎对照 1 组和子宫内膜炎诱导组。所有奶牛均喂食 2.5kg 大豆，然后在预定时间间隔内从颈静脉采集血液样本 8 小时。使用高效液相色谱系统测量血液血浆中大豆衍生异黄酮及其活性代谢物的浓度。通过荧光法测量奶牛血液血浆中的β-葡糖苷酸酶活性。在患有诱导性乳腺炎和子宫内膜炎的奶牛血液血浆中，我们发现异黄酮代谢物（黄豆苷元和对乙基苯酚）的浓度明显更高，并且与循环奶牛相比呈时间依赖性增加（P<0.05）。此外，我们注意到与对照奶牛相比，患有子宫内膜炎的奶牛血液血浆中的染料木黄酮明显减少（P<0.05）。此外，在患有子宫内膜炎的奶牛血液血浆中，我们发现与对照奶牛相比，β-葡糖苷酸酶活性增加（P<0.05）。总之，雌性动物的健康状况会影响奶牛血液血浆中异黄酮代谢物的浓度。实验性诱导的乳腺炎和子宫内膜炎增加了异黄酮的吸收、生物转化和代谢。因此，我们建议患有诱导性乳腺炎和子宫内膜炎的奶牛比健康奶牛更容易受到活性异黄酮代谢物作用的影响。
