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牛乳头瘤病毒1型和2型基因组的物理图谱。

Physical maps of bovine papillomavirus type 1 and type 2 genomes.

作者信息

Lancaster W D

出版信息

J Virol. 1979 Nov;32(2):684-7. doi: 10.1128/JVI.32.2.684-687.1979.

DOI:10.1128/JVI.32.2.684-687.1979
PMID:228086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353602/
Abstract

Physical maps of bovine papillomavirus type 1 and type 2 (BPV-1 and BPV-2) DNA were constructed from analysis of the electrophoretic mobilities of restriction endonuclease cleavage fragments from dual digests. BPV-1 DNA was sensitive to Hind III, HindIII, EcoRI, HpaI, AND BamHI, with all but HindII yielding single scissions. BPV-2 DNA was resistant to EcoRI, and HindIII had one cleavage site whereas HpaI, BamHI, and HindII yielded multiple fragments. Of four BPV-1 isolates examined, DNA from one isolate was resistant to HindIII, and another DNA isolate was resistant to BamHI. The three BPV-2 isolates examined were uniformly sensitive to the restriction endonucleases employed.

摘要

通过对双酶切产生的限制性内切酶切割片段的电泳迁移率进行分析，构建了1型和2型牛乳头瘤病毒（BPV-1和BPV-2）的物理图谱。BPV-1 DNA对Hind III、HindIII、EcoRI、HpaI和BamHI敏感，除HindII外，其余酶切均产生单一切口。BPV-2 DNA对EcoRI有抗性，HindIII有一个切割位点，而HpaI、BamHI和HindII产生多个片段。在所检测的4株BPV-1分离株中，一株分离株的DNA对HindIII有抗性，另一株DNA分离株对BamHI有抗性。所检测的3株BPV-2分离株对所用的限制性内切酶均呈一致的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/8fa31428a45e/jvirol00191-0339-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/5112a99389ab/jvirol00191-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/9c62309ae938/jvirol00191-0337-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/8fa31428a45e/jvirol00191-0339-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/5112a99389ab/jvirol00191-0337-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/9c62309ae938/jvirol00191-0337-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b275/353602/8fa31428a45e/jvirol00191-0339-a.jpg

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引用本文的文献

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Isolation of clones of hamster embryo cells transformed by the bovine papilloma virus.
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European elk papillomavirus: characterization of the genome, induction of tumors in animals, and transformation in vitro.欧洲驼鹿乳头瘤病毒：基因组特征、动物肿瘤诱导及体外转化
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3
Animal papillomaviruses.动物乳头瘤病毒。
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本文引用的文献

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J Virol. 1979 Oct;32(1):199-207. doi: 10.1128/JVI.32.1.199-207.1979.
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Hybridization of bovine papilloma virus type 1 and type 2 DNA to DNA from virus-induced hamster tumors and naturally occurring equine tumors.1型和2型牛乳头瘤病毒DNA与病毒诱导的仓鼠肿瘤及自然发生的马肿瘤的DNA杂交。
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Human papillomavirus DNA: physical map.人乳头瘤病毒DNA：物理图谱。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4810-4. doi: 10.1073/pnas.72.12.4810.