Oberly T J, Kokkino A J, Bewsey B J, Richardson K K
Toxicology Division, Lilly Research Laboratories, A Division of Eli Lilly and Company, Greenfield, IN 46140.
Mutat Res. 1990 Jun;241(2):151-9. doi: 10.1016/0165-1218(90)90119-m.
The hair-dye ingredients, HC Blue No. 1 (HCB1) and HC Blue No. 2 (HCB2), were tested for the induction of bacterial mutation using Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100; and Escherichia coli strains WP2uvrA-. In addition, both dyes were evaluated in the mouse lymphoma L5178Y TK+/- assay (MLA) for the potential to induce forward mutation. A liver homogenate (S9) prepared from Aroclor 1254-induced male Fischer 344 rats was used to provide a means for metabolic activation. HCB1 was not mutagenic in the Ames assay, but was weakly mutagenic in the MLA only in the presence of metabolic activation. In contrast, HCB2 was a strong mutagen in the Ames assay in tester strain TA98 both in the presence and absence of metabolic activation. A positive response was also noted with HCB2 in the MLA, both in the presence and absence of metabolic activation. Negative findings from the Ames assay of this study agree with other published results where an identical lot of HCB1 was used. Using the same lot, a weak positive result was observed in the MLA, however, the activation requirements and magnitude of the response were different from that of a lot evaluated by the NTP. In contrast, HCB2 appears to be both a bacterial and mammalian cell mutagen independent of lot variability.
采用鼠伤寒沙门氏菌TA1535、TA1537、TA98和TA100菌株，以及大肠杆菌WP2uvrA-菌株，对染发剂成分酸性蓝1号（HC蓝1号，HCB1）和酸性蓝2号（HC蓝2号，HCB2）进行细菌诱变性检测。此外，还通过小鼠淋巴瘤L5178Y TK+/-试验（MLA）对这两种染料诱导正向突变的可能性进行了评估。使用从经多氯联苯混合物1254诱导的雄性Fischer 344大鼠制备的肝匀浆（S9）来提供代谢活化手段。HCB1在艾姆斯试验中无致突变性，但仅在有代谢活化的情况下在MLA中具有弱致突变性。相比之下，HCB2在TA98测试菌株的艾姆斯试验中，无论有无代谢活化均为强致突变剂。在MLA中，无论有无代谢活化，HCB2也呈现阳性反应。本研究的艾姆斯试验阴性结果与使用相同批次HCB1的其他已发表结果一致。使用同一批次时，在MLA中观察到弱阳性结果，然而，活化要求和反应强度与美国国家毒理学计划评估的一个批次不同。相比之下，HCB2似乎是一种细菌和哺乳动物细胞诱变剂，与批次变异性无关。
