Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, SA 5042, Australia.
Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham Province 44150, Thailand.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1687-1691. doi: 10.1099/ijs.0.033258-0. Epub 2011 Sep 2.
A novel aerobic actinobacterium, strain EUM 273(T), was isolated from the root of a Grey Box tree (Eucalyptus microcarpa Maiden). Cells were Gram-staining-positive with well-developed substrate mycelia which were non-motile and rod-like, with coccoid elements. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed the isolate as a member of the family Promicromonosporaceae that was most closely related to Promicromonospora xylanilytica YIM 61515(T) (98.2%) and Promicromonospora vindobonensis V45(T) (98%). Chemotaxonomic data including cell wall components, major menaquinone and major fatty acids confirmed the affiliation of strain EUM 273(T) to the genus Promicromonospora. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA-DNA hybridization, allowed the genotypic and phenotypic differentiation of strain EUM 273(T) from the closest related species with validly published names. The name proposed for the novel species is Promicromonospora endophytica sp. nov. The type strain is EUM 273(T) (=DSM 23716(T)=NRRL B-24816(T)).
一株好氧放线菌，菌株 EUM 273(T)，从灰箱树（Eucalyptus microcarpa Maiden）的根部分离得到。细胞革兰氏染色阳性，具有发达的基质菌丝，无运动性，呈杆状，有球菌状的元素。基于 16S rRNA 基因序列分析的系统发育评估将该分离物置于 Promicromonosporaceae 科中，与 Promicromonospora xylanilytica YIM 61515(T)（98.2%）和 Promicromonospora vindobonensis V45(T)（98%）最为密切相关。化学分类学数据包括细胞壁成分、主要类异戊二烯醌和主要脂肪酸，证实了菌株 EUM 273(T)属于 Promicromonospora 属。基于系统发育分析的结果，包括生理和生化研究以及 DNA-DNA 杂交，允许从具有有效发表名称的最密切相关的种中对菌株 EUM 273(T)进行基因型和表型的区分。提议的新物种名称为 Promicromonospora endophytica sp. nov. 模式菌株为 EUM 273(T)=DSM 23716(T)=NRRL B-24816(T)。
