Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, D-26111 Oldenburg, Germany.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1619-1624. doi: 10.1099/ijs.0.033563-0. Epub 2011 Sep 2.
A heterotrophic, aerobic bacterium, designated strain SH6-1(T), was obtained from a seawater sample collected from the open North Sea during a phytoplankton bloom. Strain SH6-1(T) was isolated from a 10(-6) dilution culture, which indicated a high abundance of this organism in the environmental sample. 16S rRNA gene sequence comparison revealed that strain SH6-1(T) belonged to the marine Roseobacter clade (order Rhodobacterales) within the class Alphaproteobacteria. Pelagicola litoralis CL-ES2(T) was the closest phylogenetic neighbour (96.4% 16S rRNA gene sequence similarity). Cells of strain SH6-1(T) were small or elongated irregular rods. Optimal growth occurred between 20 and 25 °C and between pH 7.5 and 9.0 with peptone and yeast extract. On marine agar, the isolate formed non-pigmented, small, circular, convex colonies. For growth, cells required sodium ions and the vitamins pantothenic acid and nicotinic acid amide. The DNA G+C content was 53.8 mol%. The fatty acids (>1%) were C(10:0) 3-OH, C(16:0), C(12:1), C(12:1) 3-OH, C(18:0), C(18:1)ω7c, C(18:2) and 11-methyl C(18:1)ω7c. The polar lipid pattern indicated the presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and one unidentified phospholipid. The major respiratory lipoquinone was ubiquinone Q-10. Strain SH6-1(T) contained the genes pufLM, which code for the bacterial photosynthesis reaction centre; however, no bacteriochlorophyll a could be detected. Physiological, genotypic and phenotypic differences from P. litoralis support the description of a novel genus and species, for which we suggest the name Planktotalea frisia gen. nov., sp. nov; the type strain of the type species is SH6-1(T) (=DSM 23709(T)=LMG 25294(T)).
一株好氧异养细菌，命名为 SH6-1(T) 株，从北海浮游植物大量繁殖期间采集的海水样本中分离得到。SH6-1(T) 株从 10(-6)稀释培养物中分离得到，这表明该生物在环境样品中的丰度很高。16S rRNA 基因序列比较表明，SH6-1(T) 株属于海洋红杆菌科（Rhodobacterales 目）的α变形菌纲。Pelagicola litoralis CL-ES2(T) 是最接近的系统发育近缘种（16S rRNA 基因序列相似度为 96.4%）。SH6-1(T) 株细胞为小或长不规则棒状。最佳生长温度为 20-25°C，pH 值为 7.5-9.0，使用蛋白胨和酵母提取物。在海洋琼脂上，该分离株形成非色素、小、圆形、凸起的菌落。为了生长，细胞需要钠离子和泛酸和烟酰胺酸酰胺等维生素。DNA G+C 含量为 53.8mol%。脂肪酸（>1%）为 C(10:0) 3-OH、C(16:0)、C(12:1)、C(12:1) 3-OH、C(18:0)、C(18:1)ω7c、C(18:2) 和 11-甲基 C(18:1)ω7c。极性脂模式表明存在磷脂酰胆碱、磷脂酰甘油、一种未鉴定的胺脂和一种未鉴定的磷脂。主要呼吸类脂醌为泛醌 Q-10。SH6-1(T) 株含有编码细菌光合作用反应中心的 pufLM 基因，但未检测到细菌叶绿素 a。与 P. litoralis 的生理、基因型和表型差异支持一个新属和种的描述，我们建议使用 Planktotalea frisia gen. nov.，sp. nov. 来命名该属和种；模式种为 SH6-1(T)（=DSM 23709(T)=LMG 25294(T)）。
