Park Sooyeon, Jung Yong-Taek, Won Sung-Min, Park Ja-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2014 Sep;106(3):535-42. doi: 10.1007/s10482-014-0225-1. Epub 2014 Jul 8.
A Gram-stain-negative, aerobic, non-motile and rod-shaped or ovoid bacterial strain, GJSW-22(T), which was isolated from seawater at Geoje island in South Korea, was characterized taxonomically. Strain GJSW-22(T) was observed to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GJSW-22(T) grouped with the type strains of Thalassobius species, forming a stable cluster with the type strain of Thalassobius aestuarii (bootstrap value of 83.2 %). Strain GJSW-22(T) exhibited the highest 16S rRNA gene sequence similarity value (98.0 %) to the type strain of T. aestuarii. It exhibited 16S rRNA gene sequence similarity values of 95.6-96.1 % to the type strains of the other Thalassobius species. Strain GJSW-22(T) was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c and 11-methyl C18:1 ω7c as the major fatty acids. The major polar lipids of strain GJSW-22(T) were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G + C content of strain GJSW-22(T) is 60.3 mol % and its mean DNA-DNA relatedness value with the type strain of T. aestuarii was 23 %. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, confirmed that strain GJSW-22(T) is distinct from other Thalassobius species. On the basis of the data presented, strain GJSW-22(T) is considered to represent a novel species of the genus Thalassobius, for which the name Thalassobius aquaeponti sp. nov. is proposed. The type strain is GJSW-22(T) (=KCTC 42115(T) = NBRC 110378(T)).
从韩国巨济岛海水分离出的一株革兰氏阴性、需氧、无运动性、杆状或卵形的细菌菌株GJSW-22(T)，进行了分类学特征研究。观察到菌株GJSW-22(T)在30℃、pH 7.0 - 8.0以及2.0%（w/v）NaCl存在的条件下生长最佳。在基于16S rRNA基因序列的邻接法系统发育树中，菌株GJSW-22(T)与嗜盐栖海杆菌属（Thalassobius）的模式菌株聚在一起，与河口嗜盐栖海杆菌（Thalassobius aestuarii）模式菌株形成一个稳定的簇（自展值为83.2%）。菌株GJSW-22(T)与河口嗜盐栖海杆菌模式菌株的16S rRNA基因序列相似性值最高（98.0%）。它与其他嗜盐栖海杆菌属物种模式菌株的16S rRNA基因序列相似性值为95.6 - 96.1%。发现菌株GJSW-22(T)以Q-10作为主要泛醌，以C18:1 ω7c和11-甲基C18:1 ω7c作为主要脂肪酸。菌株GJSW-22(T)的主要极性脂类被鉴定为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的氨基脂类和一种未鉴定的脂类。菌株GJSW-22(T)的DNA G + C含量为60.3 mol%，其与河口嗜盐栖海杆菌模式菌株的平均DNA - DNA相关性值为23%。不同的表型特征，以及系统发育和化学分类学数据，证实菌株GJSW-22(T)与其他嗜盐栖海杆菌属物种不同。基于所提供的数据，菌株GJSW-22(T)被认为代表嗜盐栖海杆菌属的一个新物种，为此提出新名称河口嗜盐栖海杆菌（Thalassobius aquaeponti sp. nov.）。模式菌株为GJSW-22(T)（=KCTC 42115(T) = NBRC 110378(T)）。
