Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg, D-26111 Oldenburg, Germany.
DSMZ - German Collection of Microorganisms and Cell Cultures, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):835-843. doi: 10.1099/ijs.0.040675-0. Epub 2012 May 18.
A heterotrophic, Gram-stain-negative, aerobic bacterium, designated strain SH4-1(T), was obtained from a seawater sample collected from the southern North Sea during a phytoplankton bloom. The 16S rRNA gene sequence comparison revealed affiliation to the Roseobacter clade (class Alphaproteobacteria) with Sulfitobacter marinus SW-265(T) as the most closely related characterized strain, showing 97.2 % 16S rRNA gene sequence similarity. Calculation of phylogenetic trees based on 16S rRNA gene sequences indicated, however, that members of the genus Roseobacter, Roseobacter denitrificans Och 114(T) and Roseobacter litoralis Och 149(T) (95 % and 96 % sequence similarity, respectively) fall between strain SH4-1(T) and the Sulfitobacter cluster including Oceanibulbus indolifex HEL-45(T) (≥95.4 % sequence similarity). Cells of strain SH4-1(T) are irregular rods with at least one flagellum. Optimal growth occurred between 28 and 32 °C and at a pH between 7.0 and 8.5. Cells require the vitamin nicotinic acid amide as well as sodium ions for growth. The DNA G+C content was 55.1 mol%. The fatty acids (>1 %) comprised C10 : 0 3-OH, C12 : 1, C14 : 1 3-OH, C16 : 0, C18 : 0, C18 : 2, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The polar lipid pattern indicated the presence of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid, one unidentified phospholipid and one other unidentified lipid. On the basis of phenotypic, chemotaxonomic and phylogenetic differences, strain SH4-1(T) represents a novel species in a new genus within the family Rhodobacteraceae, for which we propose the name Pelagimonas varians gen. nov., sp. nov. The type strain of the type species is SH4-1(T) ( = DSM 23678(T) = LMG 26343(T) = CIP 110297(T)).
一株异养、革兰氏阴性、需氧细菌，被命名为 SH4-1(T) 株，是从北海南部浮游植物大量繁殖期间采集的海水样本中分离得到的。16S rRNA 基因序列比较表明，该菌属于玫瑰杆菌群（α-变形菌纲），与 Sulfitobacter marinus SW-265(T) 最为密切相关，同源性为 97.2%。然而，基于 16S rRNA 基因序列构建的系统发育树表明，玫瑰杆菌属的成员，Roseobacter denitrificans Och 114(T) 和 Roseobacter litoralis Och 149(T)（分别为 95%和 96%的序列相似性）位于 SH4-1(T) 株和包括 Oceanibulbus indolifex HEL-45(T)（≥95.4%的序列相似性）在内的 Sulfitobacter 群之间。SH4-1(T) 株的细胞为不规则杆状，至少有一个鞭毛。最适生长温度为 28-32°C，最适 pH 值为 7.0-8.5。细胞生长需要烟酸酰胺和钠离子。DNA G+C 含量为 55.1mol%。主要脂肪酸（＞1%）为 C10:0 3-OH、C12:1、C14:1 3-OH、C16:0、C18:0、C18:2、C18:1ω7c 和 11-甲基 C18:1ω7c。极性脂分析表明存在磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰单甲基乙醇胺、一种未鉴定的胺脂、一种未鉴定的磷脂和一种未鉴定的脂类。基于表型、化学分类和系统发育差异，SH4-1(T) 株代表了 Rhodobacteraceae 科一个新属中的一个新种，我们将其命名为 Pelagimonas varians gen. nov.，sp. nov.。模式种为 SH4-1(T)（=DSM 23678(T)=LMG 26343(T)=CIP 110297(T)）。
