13C-NMR 定量测定水中 LewisX 羟基的质子交换。
13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water.
机构信息
Structural Glycobiology Team, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan.
出版信息
Chem Commun (Camb). 2011 Oct 14;47(38):10800-2. doi: 10.1039/c1cc13310a. Epub 2011 Sep 5.
PMID:21892456
Abstract
NMR-based analysis of glycans by directly observing hydroxyl protons has been difficult because of their inherently fast exchange with water. We observed hydroxyl proton exchanges in a LewisX-LewisX interaction by using deuterium isotope shifts on (13)C-NMR. This strategy is suitable for analyzing weak interactions by identifying involved protons.
摘要
通过直接观察羟基质子对聚糖进行基于 NMR 的分析一直很困难，因为它们与水的交换速度非常快。我们通过 (13)C-NMR 的氘同位素位移观察到了 LewisX-LewisX 相互作用中的羟基质子交换。该策略通过鉴定相关质子，适合用于分析弱相互作用。
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