Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
Chemosphere. 2011 Oct;85(3):548-52. doi: 10.1016/j.chemosphere.2011.08.021. Epub 2011 Sep 3.
To examine the bioremediation potential of Mortierella sp. strain W8 in endosulfan contaminated soil, the fungus was inoculated into sterilized and unsterilized soil spiked with endosulfan. Wheat bran and cane molasses were used as substrates to understand the influence of different organic materials on the degradation of endosulfan in soil. Strain W8 degraded α- and β-endosulfan in both sterilized and unsterilized soil. In unsterilized soil with wheat bran+W8, α- and β- endosulfan were degraded by approximately 80% and 50%, respectively after 28 d incubation against the initial endosulfan concentration (3 mg kg(-1) dw). The corresponding values for α- and β-endosulfan degradation with wheat bran only were 50% and 3%. Endosulfan diol metabolite was detected after 14 d incubation in wheat bran+W8 whereas it was not found with wheat bran only. Production of endosulfan sulfate, the main metabolite of endosulfan, was suppressed with wheat bran+W8 treatment compared with wheat bran only. It was demonstrated that wheat bran is a more suitable substrate for strain W8 than cane molasses. Wheat bran+W8 is a superior fungus and substrate mix for bioremediation in soil contaminated with endosulfan.
为了研究枝顶孢霉（Mortierella sp.）菌株 W8 对污染土壤中硫丹的生物修复潜力，将该真菌接种到添加了硫丹的灭菌和未灭菌土壤中。麦麸和甘蔗糖蜜被用作底物，以了解不同有机材料对土壤中硫丹降解的影响。菌株 W8 在灭菌和未灭菌土壤中均可降解α-和β-硫丹。在添加麦麸和 W8 的未灭菌土壤中，α-和β-硫丹在 28 天的培养后分别降解了约 80%和 50%，而初始硫丹浓度（3mgkg(-1)dw）分别降解了约 80%和 50%。仅用麦麸时，α-和β-硫丹的降解率分别为 50%和 3%。在麦麸+W8 中培养 14 天后检测到了硫丹二醇代谢物，但在仅用麦麸时未检测到。与仅用麦麸相比，麦麸+W8 处理抑制了硫丹硫酸盐的产生，硫丹硫酸盐是硫丹的主要代谢物。结果表明，与甘蔗糖蜜相比，麦麸是菌株 W8 的更适宜底物。麦麸+W8 是生物修复受硫丹污染土壤的优良真菌和底物组合。
