Fuster Gemma, Almendro Vanessa, Fontes-Oliveira Cibely Cristine, Toledo Míriam, Costelli Paola, Busquets Sílvia, López-Soriano Francisco J, Argilés Josep M
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Cancer Research Group, Universitat de Barcelona, Diagonal 645, 08028, Barcelona, Spain.
Lipids. 2011 Nov;46(11):1033-42. doi: 10.1007/s11745-011-3594-5. Epub 2011 Sep 6.
Interleukin-15 (IL-15) is an anabolic factor for skeletal muscle and several reports have described its important role as a regulator of energy homeostasis. In this study, we analyzed the effects of IL-15 on adipocyte differentiation using the 3T3-L1 preadipose cell line. The data show that IL-15 tends to reduce the rate of adipocyte proliferation, induces apoptosis, and partially stops differentiation. The signaling molecules behind these actions of the cytokine on adipose cells are: p42/p44 MAPK (which seem to be associated with the reduced rate of proliferation induced by the cytokine), STAT5 (which is related to the actions of IL-15 on differentiation), and SAPK/JNK (which are related to the increased apoptosis induced by IL-15). In conclusion, using the 3T3-L1 adipocyte cell line, the results presented here show that IL-15 exerts important effects on differentiation, proliferation and apoptosis. Altogether, the results presented here reinforce the idea that IL-15 is an important mediator that regulates adipose size and, therefore, the role of the cytokine in affecting body weight and obesity deserves additional studies.
白细胞介素-15（IL-15）是骨骼肌的一种合成代谢因子，已有多篇报道描述了其作为能量稳态调节因子的重要作用。在本研究中，我们使用3T3-L1前脂肪细胞系分析了IL-15对脂肪细胞分化的影响。数据显示，IL-15倾向于降低脂肪细胞增殖速率，诱导细胞凋亡，并部分阻止分化。细胞因子对脂肪细胞这些作用背后的信号分子有：p42/p44 MAPK（似乎与细胞因子诱导的增殖速率降低有关）、STAT5（与IL-15对分化的作用有关）和SAPK/JNK（与IL-15诱导的细胞凋亡增加有关）。总之，使用3T3-L1脂肪细胞系，本文呈现的结果表明IL-15对分化、增殖和凋亡具有重要影响。总体而言，本文呈现的结果强化了IL-15是调节脂肪大小的重要介质这一观点，因此，该细胞因子在影响体重和肥胖方面的作用值得进一步研究。
