Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture of the People's Republic of China, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, Guangdong, People's Republic of China.
Mol Biol Rep. 2012 Apr;39(4):4359-65. doi: 10.1007/s11033-011-1223-2. Epub 2011 Sep 6.
Insulin-like growth factor II (IGF-II) is involved in the regulation of somatic growth and metabolism in many fishes. IGF-II is an important candidate gene for growth traits in fishes and its polymorphisms were associated with the growth traits. The aim of this study is to screen single nucleotide polymorphisms (SNPs) of the largemouth bass (Micropterus salmoides) IGF-II gene and to analyze potential association between IGF-II gene polymorphisms and growth traits in largemouth bass. Four SNPs (C127T, T1012G, C1836T and C1861T) were detected and verified by DNA sequencing in the largemouth bass IGF-II gene. These SNPs were found to organize into seven haplotypes, which formed 13 diplotypes (haplotype pairs). Association analysis showed that four individual SNPs were not significantly associated with growth traits. Significant associations were, however, noted between diplotypes and growth traits (P < 0.05). The fish with H1H3 (CTCC/CGCC) and H1H5 (CTCC/TTTT) had greater body weight than those with H1H1 (CTCC/CTCC), H1H2 (CTCC/TGTT) and H4H4 (TGCT/TGCT/) did. Our data suggest a significant association between genetic variations in the largemouth bass IGF-II gene and growth traits. IGF-II SNPs could be used as potential genetic markers in future breeding programs of largemouth bass.
胰岛素样生长因子 II（IGF-II）参与许多鱼类的体生长和代谢调节。IGF-II 是鱼类生长性状的重要候选基因，其多态性与生长性状有关。本研究旨在筛选大口黑鲈（Micropterus salmoides）IGF-II 基因的单核苷酸多态性（SNPs），并分析 IGF-II 基因多态性与大口黑鲈生长性状的潜在关联。在大口黑鲈 IGF-II 基因中检测到并通过 DNA 测序验证了 4 个 SNP（C127T、T1012G、C1836T 和 C1861T）。这些 SNP 分为 7 种单倍型，形成 13 种二倍型（单倍型对）。关联分析表明，4 个个体 SNP 与生长性状无显著关联。然而，二倍型与生长性状之间存在显著关联（P<0.05）。与 H1H1（CTCC/CTCC）和 H1H2（CTCC/TGTT）相比，H1H3（CTCC/CGCC）和 H1H5（CTCC/TTTT）的鱼体重更大。我们的数据表明，大口黑鲈 IGF-II 基因的遗传变异与生长性状之间存在显著关联。IGF-II SNPs 可作为大口黑鲈未来育种计划的潜在遗传标记。
