Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing 100190, PR China.
J Am Chem Soc. 2011 Oct 5;133(39):15284-7. doi: 10.1021/ja205712a. Epub 2011 Sep 12.
A novel strategy of dual steric hindrance, which was obtained by Janus modification of gold nanoparticles (Au NPs) and volume exclusion of DNA, was adopted to prepare mono-DNA-modified Au NPs. The yield of mono-DNA-functionalized Au NPs significantly improved from 44 to 70% in the reaction between Au NPs and thiolated DNA. Furthermore, the specificity of mono-DNA-functionalized Au NPs was enhanced from 57 to 95%. The as-prepared Au NPs without postsynthetic treatment showed good controllability in self-assembly fabrication of complex nanostructures.
采用金纳米粒子（Au NPs）的双面位阻修饰和 DNA 的体积排除的新策略，制备了单 DNA 修饰的 Au NPs。Au NPs 与巯基化 DNA 反应时，单 DNA 功能化 Au NPs 的产率从 44%显著提高到 70%。此外，单 DNA 功能化 Au NPs 的特异性从 57%提高到 95%。未经后期合成处理的所制备的 Au NPs 显示出在复杂纳米结构的自组装制造中具有良好的可控性。
